A method is described for the production of T-cell lines and clones specific for solubilized Trichinella spiralis antigens. hese T cells are antigen-specific and do not respond to challenge with a third party antigen (lysozyme). The proliferation responses of the cloned T cells are specifically inhibited by anti-I-E but not by anti I-A subregion monoclonal reagents. The inhibition patterns obtained are consistent with cis-gene complementation in B10.K cells involving the Ekβ-chain and the Ek α-chain of the I-E molecule. Inhibition is obtained with an Ekβ-specific monoclonal antibody (H9-14.8) but not with an Akβ-specific monoclonal antibody (10-2.16). Inhibition was also observed with Ia.7-specific (H40-242) or Ia.22-specific (17-3-3) monoclonal antibodies. The inhibition patterns were confirmed by antigen presentation experiments using recombinant inbred mice. Only B 10.K (EkβEkα spleen cells and not B 10.A(5R) (EbβEkα) or B10.S(9R) (EsβEkα) spleen cells could effectively present T. spiralis antigens. The role of "hybrid" Ia molecules in the immune response to T. spiralis is discussed.
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