Cloned HBV DNA causes hepatitis in chimpanzees

H. Will, Roberto Cattaneo, H. G. Koch, G. Darai, H. Schaller, H. Schellekens, P. M. van Eerd, F. Deinhardt

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Most of our knowledge of the structure and function of the hepatitis B virus (HBV) genome comes from the analysis of the viral DNA sequences cloned in bacteria. Because the physical state of cloned HBV DNA differs from HBV DNA encapsidated in the virion - for example, it lacks the nick-gap structure, and a covalently linked protein - the question arises as to whether it can initiate HBV replication in vitro or in vivo. We describe here the development of typical acute viral hepatitis, and the detection of HBV-specific DNA sequences in the serum and liver, in a chimpanzee inoculated with cloned HBV DNA. This demonstrates that neither the virion proteins nor the nick-gap structure of the virion DNA are needed for the initiation of replication of HBV in vivo.

Original languageEnglish (US)
Pages (from-to)740-742
Number of pages3
JournalNature
Volume299
Issue number5885
DOIs
StatePublished - 1982
Externally publishedYes

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Pan troglodytes
Hepatitis B virus
Hepatitis
DNA
Virion
Viral DNA
Virus Replication
DNA Sequence Analysis
Proteins
Genome
Bacteria
Liver
Serum

ASJC Scopus subject areas

  • General

Cite this

Will, H., Cattaneo, R., Koch, H. G., Darai, G., Schaller, H., Schellekens, H., ... Deinhardt, F. (1982). Cloned HBV DNA causes hepatitis in chimpanzees. Nature, 299(5885), 740-742. https://doi.org/10.1038/299740a0

Cloned HBV DNA causes hepatitis in chimpanzees. / Will, H.; Cattaneo, Roberto; Koch, H. G.; Darai, G.; Schaller, H.; Schellekens, H.; van Eerd, P. M.; Deinhardt, F.

In: Nature, Vol. 299, No. 5885, 1982, p. 740-742.

Research output: Contribution to journalArticle

Will, H, Cattaneo, R, Koch, HG, Darai, G, Schaller, H, Schellekens, H, van Eerd, PM & Deinhardt, F 1982, 'Cloned HBV DNA causes hepatitis in chimpanzees', Nature, vol. 299, no. 5885, pp. 740-742. https://doi.org/10.1038/299740a0
Will H, Cattaneo R, Koch HG, Darai G, Schaller H, Schellekens H et al. Cloned HBV DNA causes hepatitis in chimpanzees. Nature. 1982;299(5885):740-742. https://doi.org/10.1038/299740a0
Will, H. ; Cattaneo, Roberto ; Koch, H. G. ; Darai, G. ; Schaller, H. ; Schellekens, H. ; van Eerd, P. M. ; Deinhardt, F. / Cloned HBV DNA causes hepatitis in chimpanzees. In: Nature. 1982 ; Vol. 299, No. 5885. pp. 740-742.
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