Cloned HBV DNA causes hepatitis in chimpanzees

Hans Will; Roberto Cattaneo; Hans Georg Koch; Gholamreza Darai; Heinz Schaller; Hǔub Schellekens; Peer M.C.A. Van Eerd; Friedrich Deinhardt

doi:10.1038/299740a0

Cloned HBV DNA causes hepatitis in chimpanzees

Hans Will, Roberto Cattaneo, Hans Georg Koch, Gholamreza Darai, Heinz Schaller, Hǔub Schellekens, Peer M.C.A. Van Eerd, Friedrich Deinhardt

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

Most of our knowledge of the structure and function of the hepatitis B virus (HBV) genome comes from the analysis of the viral DNA sequences cloned in bacteria^1-3. Because the physical state of cloned HBV DNA differs from HBV DNA encapsidated in the virion - for example, it lacks the nick-gap structure⁴, and a covalently linked protein⁵ - the question arises as to whether it can initiate HBV replication in vitro ⁶ or in vivo. We describe here the development of typical acute viral hepatitis, and the detection of HBV-specific DNA sequences in the serum and liver, in a chimpanzee inoculated with cloned HBV DNA. This demonstrates that neither the virion proteins nor the nick-gap structure of the virion DNA are needed for the initiation of replication of HBV in vivo.

Original language	English (US)
Pages (from-to)	740-742
Number of pages	3
Journal	Nature
Volume	299
Issue number	5885
DOIs	https://doi.org/10.1038/299740a0
State	Published - 1982

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title = "Cloned HBV DNA causes hepatitis in chimpanzees",

abstract = "Most of our knowledge of the structure and function of the hepatitis B virus (HBV) genome comes from the analysis of the viral DNA sequences cloned in bacteria1-3. Because the physical state of cloned HBV DNA differs from HBV DNA encapsidated in the virion - for example, it lacks the nick-gap structure4, and a covalently linked protein5 - the question arises as to whether it can initiate HBV replication in vitro 6 or in vivo. We describe here the development of typical acute viral hepatitis, and the detection of HBV-specific DNA sequences in the serum and liver, in a chimpanzee inoculated with cloned HBV DNA. This demonstrates that neither the virion proteins nor the nick-gap structure of the virion DNA are needed for the initiation of replication of HBV in vivo.",

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T1 - Cloned HBV DNA causes hepatitis in chimpanzees

AU - Will, Hans

AU - Cattaneo, Roberto

AU - Koch, Hans Georg

AU - Darai, Gholamreza

AU - Schaller, Heinz

AU - Schellekens, Hǔub

AU - Van Eerd, Peer M.C.A.

AU - Deinhardt, Friedrich

PY - 1982

Y1 - 1982

N2 - Most of our knowledge of the structure and function of the hepatitis B virus (HBV) genome comes from the analysis of the viral DNA sequences cloned in bacteria1-3. Because the physical state of cloned HBV DNA differs from HBV DNA encapsidated in the virion - for example, it lacks the nick-gap structure4, and a covalently linked protein5 - the question arises as to whether it can initiate HBV replication in vitro 6 or in vivo. We describe here the development of typical acute viral hepatitis, and the detection of HBV-specific DNA sequences in the serum and liver, in a chimpanzee inoculated with cloned HBV DNA. This demonstrates that neither the virion proteins nor the nick-gap structure of the virion DNA are needed for the initiation of replication of HBV in vivo.

AB - Most of our knowledge of the structure and function of the hepatitis B virus (HBV) genome comes from the analysis of the viral DNA sequences cloned in bacteria1-3. Because the physical state of cloned HBV DNA differs from HBV DNA encapsidated in the virion - for example, it lacks the nick-gap structure4, and a covalently linked protein5 - the question arises as to whether it can initiate HBV replication in vitro 6 or in vivo. We describe here the development of typical acute viral hepatitis, and the detection of HBV-specific DNA sequences in the serum and liver, in a chimpanzee inoculated with cloned HBV DNA. This demonstrates that neither the virion proteins nor the nick-gap structure of the virion DNA are needed for the initiation of replication of HBV in vivo.

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Cloned HBV DNA causes hepatitis in chimpanzees

Abstract

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