@article{581070f7e48d4b00961448a461904c57,
title = "Clonal analysis via barcoding reveals diverse growth and differentiation of transplanted mouse and human mammary stem cells",
abstract = "Cellular barcoding offers a powerful approach to characterize the growth and differentiation activity of large numbers of cotransplanted stem cells. Here, we describe a lentiviral genomic-barcoding and analysis strategy and its use to compare the clonal outputs of transplants of purified mouse and human basal mammary epithelial cells. We found that both sources of transplanted cells produced many bilineage mammary epithelial clones in primary recipients, although primary clones containing only one detectable mammary lineage were also common. Interestingly, regardless of the species of origin, many clones evident in secondary recipients were not detected in the primary hosts, and others that were changed from appearing luminal-restricted to appearing bilineage. This barcoding methodology has thus revealed conservation between mice and humans of a previously unknown diversity in the growth and differentiation activities of their basal mammary epithelial cells stimulated to grow in transplanted hosts.",
author = "Nguyen, {Long V.} and Maisam Makarem and Annaick Carles and Michelle Moksa and Nagarajan Kannan and Pawan Pandoh and Peter Eirew and Tomo Osako and Melanie Kardel and Cheung, {Alice M.S.} and William Kennedy and Kane Tse and Thomas Zeng and Yongjun Zhao and Humphries, {R. Keith} and Samuel Aparicio and Eaves, {Connie J.} and Martin Hirst",
note = "Funding Information: The authors thank J. Emerman, U. Kuuske, J. Sproul, and N. Van Laeken for assistance in obtaining reduction mammoplasty samples; D. Ko and W. Xu (Flow Cytometry Facility, Terry Fox Laboratory) for assistance with flow cytometry; G. Edin, M. Hale, D. Wilkinson, and Patty Rosten for excellent technical support; and T. MacDonald for assistance with rodent husbandry. This work was supported by grants from the Canadian Breast Cancer Research Alliance funded by the Canadian Cancer Society (CCS) and the Canadian Breast Cancer Foundation (CBCF), in partnership with Avon Canada, the Canadian Breast Cancer Network, the CCS, the Canadian Institutes of Health Research (CIHR), the Stem Cell Network, a Terry Fox Foundation program project grant (TFF-122869), Health Canada, and the Public Health Agency of Canada. L.V.N. received a Vanier Canada Graduate Scholarship from the CIHR, M.M. and M.K. received Doctoral Canada Graduate Scholarships from CIHR, N.K. received a postdoctoral fellowship from the BC and Yukon Division of the CBCF, and T.O. is supported by a CIHR pathology training fellowship. S.A. is supported by a Canada Research Chair in Molecular Oncology. ",
year = "2014",
month = feb,
day = "6",
doi = "10.1016/j.stem.2013.12.011",
language = "English (US)",
volume = "14",
pages = "253--263",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "2",
}