Clinical performance of parathyroid hormone immunometric assays

P. C. Kao, J. A. Van Heerden, C. S. Grant, G. G. Klee, Sundeep Khosla

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Three immunometric assays of parathyroid hormone (PTH)-a commercial immunoradiometric assay, an in-house immunoradiometric assay, and an immunochemiluminometric assay-were evaluated in 50 patients with surgically proven primary hyperparathyroidism. Of these patients, 43 had increased values with the commercial assay (sensitivity, 86%), whereas 45 patients had increased concentrations with both the in-house immunoradiometric and the in- house immunochemiluminometric assays (sensitivities, 90%). Because of the results of this comparison study, we confidently chose the immunochemiluminometric assay as our routine assay; this assay was evaluated retrospectively in 361 patients with surgically proven primary hyperparathyroidism. In 45 patients, PTH values were below the upper limit of normal (sensitivity, 88%). The results indicate that the sensitivities of current immunometric assays are approximately 90%. Twenty patients who had hypercalcemia associated with malignant involvement were assessed with the immunochemiluminometric assay. Of these 20 patients, 19 had subnormal PTH values, and 1 had a value within the normal range. In contrast, in the past, PTH values determined with radioimmunoassays have often been in the normal range for such patients. Thus, an immunometric PTH assay is superior to a radioimmunoassay in the differential diagnosis of hypercalcemia associated with malignant disease.

Original languageEnglish (US)
Pages (from-to)637-645
Number of pages9
JournalMayo Clinic Proceedings
Volume67
Issue number7
StatePublished - 1992

Fingerprint

Parathyroid Hormone
Immunoradiometric Assay
Primary Hyperparathyroidism
Hypercalcemia
Radioimmunoassay
Reference Values
Differential Diagnosis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kao, P. C., Van Heerden, J. A., Grant, C. S., Klee, G. G., & Khosla, S. (1992). Clinical performance of parathyroid hormone immunometric assays. Mayo Clinic Proceedings, 67(7), 637-645.

Clinical performance of parathyroid hormone immunometric assays. / Kao, P. C.; Van Heerden, J. A.; Grant, C. S.; Klee, G. G.; Khosla, Sundeep.

In: Mayo Clinic Proceedings, Vol. 67, No. 7, 1992, p. 637-645.

Research output: Contribution to journalArticle

Kao, PC, Van Heerden, JA, Grant, CS, Klee, GG & Khosla, S 1992, 'Clinical performance of parathyroid hormone immunometric assays', Mayo Clinic Proceedings, vol. 67, no. 7, pp. 637-645.
Kao PC, Van Heerden JA, Grant CS, Klee GG, Khosla S. Clinical performance of parathyroid hormone immunometric assays. Mayo Clinic Proceedings. 1992;67(7):637-645.
Kao, P. C. ; Van Heerden, J. A. ; Grant, C. S. ; Klee, G. G. ; Khosla, Sundeep. / Clinical performance of parathyroid hormone immunometric assays. In: Mayo Clinic Proceedings. 1992 ; Vol. 67, No. 7. pp. 637-645.
@article{10a43adcaad04dff9fc1116c3cfb7563,
title = "Clinical performance of parathyroid hormone immunometric assays",
abstract = "Three immunometric assays of parathyroid hormone (PTH)-a commercial immunoradiometric assay, an in-house immunoradiometric assay, and an immunochemiluminometric assay-were evaluated in 50 patients with surgically proven primary hyperparathyroidism. Of these patients, 43 had increased values with the commercial assay (sensitivity, 86{\%}), whereas 45 patients had increased concentrations with both the in-house immunoradiometric and the in- house immunochemiluminometric assays (sensitivities, 90{\%}). Because of the results of this comparison study, we confidently chose the immunochemiluminometric assay as our routine assay; this assay was evaluated retrospectively in 361 patients with surgically proven primary hyperparathyroidism. In 45 patients, PTH values were below the upper limit of normal (sensitivity, 88{\%}). The results indicate that the sensitivities of current immunometric assays are approximately 90{\%}. Twenty patients who had hypercalcemia associated with malignant involvement were assessed with the immunochemiluminometric assay. Of these 20 patients, 19 had subnormal PTH values, and 1 had a value within the normal range. In contrast, in the past, PTH values determined with radioimmunoassays have often been in the normal range for such patients. Thus, an immunometric PTH assay is superior to a radioimmunoassay in the differential diagnosis of hypercalcemia associated with malignant disease.",
author = "Kao, {P. C.} and {Van Heerden}, {J. A.} and Grant, {C. S.} and Klee, {G. G.} and Sundeep Khosla",
year = "1992",
language = "English (US)",
volume = "67",
pages = "637--645",
journal = "Mayo Clinic Proceedings",
issn = "0025-6196",
publisher = "Elsevier Science",
number = "7",

}

TY - JOUR

T1 - Clinical performance of parathyroid hormone immunometric assays

AU - Kao, P. C.

AU - Van Heerden, J. A.

AU - Grant, C. S.

AU - Klee, G. G.

AU - Khosla, Sundeep

PY - 1992

Y1 - 1992

N2 - Three immunometric assays of parathyroid hormone (PTH)-a commercial immunoradiometric assay, an in-house immunoradiometric assay, and an immunochemiluminometric assay-were evaluated in 50 patients with surgically proven primary hyperparathyroidism. Of these patients, 43 had increased values with the commercial assay (sensitivity, 86%), whereas 45 patients had increased concentrations with both the in-house immunoradiometric and the in- house immunochemiluminometric assays (sensitivities, 90%). Because of the results of this comparison study, we confidently chose the immunochemiluminometric assay as our routine assay; this assay was evaluated retrospectively in 361 patients with surgically proven primary hyperparathyroidism. In 45 patients, PTH values were below the upper limit of normal (sensitivity, 88%). The results indicate that the sensitivities of current immunometric assays are approximately 90%. Twenty patients who had hypercalcemia associated with malignant involvement were assessed with the immunochemiluminometric assay. Of these 20 patients, 19 had subnormal PTH values, and 1 had a value within the normal range. In contrast, in the past, PTH values determined with radioimmunoassays have often been in the normal range for such patients. Thus, an immunometric PTH assay is superior to a radioimmunoassay in the differential diagnosis of hypercalcemia associated with malignant disease.

AB - Three immunometric assays of parathyroid hormone (PTH)-a commercial immunoradiometric assay, an in-house immunoradiometric assay, and an immunochemiluminometric assay-were evaluated in 50 patients with surgically proven primary hyperparathyroidism. Of these patients, 43 had increased values with the commercial assay (sensitivity, 86%), whereas 45 patients had increased concentrations with both the in-house immunoradiometric and the in- house immunochemiluminometric assays (sensitivities, 90%). Because of the results of this comparison study, we confidently chose the immunochemiluminometric assay as our routine assay; this assay was evaluated retrospectively in 361 patients with surgically proven primary hyperparathyroidism. In 45 patients, PTH values were below the upper limit of normal (sensitivity, 88%). The results indicate that the sensitivities of current immunometric assays are approximately 90%. Twenty patients who had hypercalcemia associated with malignant involvement were assessed with the immunochemiluminometric assay. Of these 20 patients, 19 had subnormal PTH values, and 1 had a value within the normal range. In contrast, in the past, PTH values determined with radioimmunoassays have often been in the normal range for such patients. Thus, an immunometric PTH assay is superior to a radioimmunoassay in the differential diagnosis of hypercalcemia associated with malignant disease.

UR - http://www.scopus.com/inward/record.url?scp=0026762775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026762775&partnerID=8YFLogxK

M3 - Article

C2 - 1434896

AN - SCOPUS:0026762775

VL - 67

SP - 637

EP - 645

JO - Mayo Clinic Proceedings

JF - Mayo Clinic Proceedings

SN - 0025-6196

IS - 7

ER -