Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model

Henri G. Colt, Philippe Astoul, Xiaoen Wang, Eunhee S. Yi, Christian Boutin, Robert M. Hoffman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Malignant pleural mesothelioma is an aggressive tumor that is essentially unresponsive to standard medical and surgical therapies. Little is actually known about its biologic response to therapeutic interventions, in part because of a lack of a "patient-like" animal tumor model. Most experimental models thus far have been derived from inhalation or inoculation of asbestos fibers into animal subjects or by subcutaneous transplantation of human mesothelial cell lines into nude mice. These models are not representative of clinical malignant pleural mesothelioma. In this report, an animal model of human pleural malignant mesothelioma obtained by orthotopic transplantation of intact pleural tumor tissue into athymic nude mice is described. Pleural tumor obtained by thoracoscopy from a patient with epithelial-type malignant pleural mesothelioma was implanted as intact tissue by surgical orthotopic implantation (SOI) into the right pleural cavity of nude mice. Animals were sacrificed when moribund or 6 months after implantation. Tumor growth and regional spread in the mice evaluated at postmortem examination mimicked the clinical pattern of progression of human disease. Histologic findings and the immunohistochemical profile were similar to those demonstrated on examination of thoracoscopic parietal pleural biopsy specimens and post-mortem examination of the original patient's tumor. This "patient-like" nude mouse model of epithelial-type malignant pleural mesothelioma, phenotypically similar to the original human tumor, should facilitate future investigation of tumorigenesis and metastatic potential of this neoplasm. The model should serve as a basis for assessing the impact of experimental and existing therapy on malignant mesothelioma.

Original languageEnglish (US)
Pages (from-to)633-640
Number of pages8
JournalAnticancer Research
Volume16
Issue number2
StatePublished - 1996
Externally publishedYes

Fingerprint

Nude Mice
Transplants
Neoplasms
Autopsy
Animal Models
Transplantation
Pleural Cavity
Thoracoscopy
Investigational Therapies
Malignant Mesothelioma
Asbestos
Inhalation
Disease Progression
Carcinogenesis
Theoretical Models
Biopsy
Cell Line
Therapeutics
Growth

Keywords

  • Epithelial-type malignant pleural mesothelioma
  • Human
  • Orthotopic-transplant nude mouse model

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Colt, H. G., Astoul, P., Wang, X., Yi, E. S., Boutin, C., & Hoffman, R. M. (1996). Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model. Anticancer Research, 16(2), 633-640.

Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model. / Colt, Henri G.; Astoul, Philippe; Wang, Xiaoen; Yi, Eunhee S.; Boutin, Christian; Hoffman, Robert M.

In: Anticancer Research, Vol. 16, No. 2, 1996, p. 633-640.

Research output: Contribution to journalArticle

Colt, HG, Astoul, P, Wang, X, Yi, ES, Boutin, C & Hoffman, RM 1996, 'Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model', Anticancer Research, vol. 16, no. 2, pp. 633-640.
Colt, Henri G. ; Astoul, Philippe ; Wang, Xiaoen ; Yi, Eunhee S. ; Boutin, Christian ; Hoffman, Robert M. / Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model. In: Anticancer Research. 1996 ; Vol. 16, No. 2. pp. 633-640.
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