Clinical comparison of isolator, septi-chek, nonvented tryptic soy broth, and direct agar plating combined with thioglycolate broth for diagnosing spontaneous bacterial peritonitis

J. Eileen Hay, Franklin R. Cockerill, Dorothy Kaese, Emily A. Vetter, Peter C. Wollan, Jorge Rakela, Mark P. Wilhelm

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Spontaneous bacterial peritonitis is a life-threatening complication of cirrhotic ascites. Optimal patient management depends on the isolation of the causal organism from ascitic fluid. To evaluate culture techniques for the diagnosis of spontaneous bacterial peritonitis, we prospectively compared three blood culture systems, the Isolator system, a lysis-centrifugation system, the Septi-Chek system, a biphasic culture system, and a nonvented tryptic soy broth system, all inoculated at the bedside, and our standard method of direct inoculation of specimens after transport to the laboratory onto agar plates and into thioglycolate broth. The results showed that the Septi-Chek and nonvented tryptic soy broth systems each recovered statistically significantly more pathogens than either the Isolator system (P = 0.0084) or the standard method (P = 0.00098). The Isolator system recovered more pathogens than the standard plate method, but this difference was not statistically significant. Both the Isolator system and the standard plate method recovered more contaminating microorganisms than the Septi-Chek or nonvented tryptic soy broth system. The Isolator system required the most processing time compared with the processing times required by any other method.

Original languageEnglish (US)
Pages (from-to)34-37
Number of pages4
JournalJournal of Clinical Microbiology
Volume34
Issue number1
StatePublished - 1996

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Thioglycolates
Peritonitis
Agar
Culture Techniques
Ascitic Fluid
Centrifugation
Ascites
trypticase-soy broth

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Clinical comparison of isolator, septi-chek, nonvented tryptic soy broth, and direct agar plating combined with thioglycolate broth for diagnosing spontaneous bacterial peritonitis. / Eileen Hay, J.; Cockerill, Franklin R.; Kaese, Dorothy; Vetter, Emily A.; Wollan, Peter C.; Rakela, Jorge; Wilhelm, Mark P.

In: Journal of Clinical Microbiology, Vol. 34, No. 1, 1996, p. 34-37.

Research output: Contribution to journalArticle

Eileen Hay, J. ; Cockerill, Franklin R. ; Kaese, Dorothy ; Vetter, Emily A. ; Wollan, Peter C. ; Rakela, Jorge ; Wilhelm, Mark P. / Clinical comparison of isolator, septi-chek, nonvented tryptic soy broth, and direct agar plating combined with thioglycolate broth for diagnosing spontaneous bacterial peritonitis. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 1. pp. 34-37.
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abstract = "Spontaneous bacterial peritonitis is a life-threatening complication of cirrhotic ascites. Optimal patient management depends on the isolation of the causal organism from ascitic fluid. To evaluate culture techniques for the diagnosis of spontaneous bacterial peritonitis, we prospectively compared three blood culture systems, the Isolator system, a lysis-centrifugation system, the Septi-Chek system, a biphasic culture system, and a nonvented tryptic soy broth system, all inoculated at the bedside, and our standard method of direct inoculation of specimens after transport to the laboratory onto agar plates and into thioglycolate broth. The results showed that the Septi-Chek and nonvented tryptic soy broth systems each recovered statistically significantly more pathogens than either the Isolator system (P = 0.0084) or the standard method (P = 0.00098). The Isolator system recovered more pathogens than the standard plate method, but this difference was not statistically significant. Both the Isolator system and the standard plate method recovered more contaminating microorganisms than the Septi-Chek or nonvented tryptic soy broth system. The Isolator system required the most processing time compared with the processing times required by any other method.",
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