Cleavage of a specific bond in troponin C by thrombin

P. C. Leavis; S. Rosenfeld; R. C. Lu

doi:10.1016/0005-2795(78)90094-6

Cleavage of a specific bond in troponin C by thrombin

P. C. Leavis, S. Rosenfeld, R. C. Lu

Hematology / Oncology / Cancer Center / Breast Clinic

Research output: Contribution to journal › Article › peer-review

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Abstract

Limited proteolysis of rabbit skeletal troponin C with bovine thrombin yielded two fragments, TH1 (M_r = 11 000) containing Ca²⁺ binding regions I-III and TH2 (M_r = 6000) containing region IV. Determination of the partial sequences of the fragments established the site of cleavage at Arg₁₂₀-Ala₁₂₁. Secondary cleavage by thrombin at other arginyl or lysyl residues in troponin C was ruled out by the sequence data and by the amino acid compositions of the two fragments.

Original language	English (US)
Pages (from-to)	281-286
Number of pages	6
Journal	BBA - Protein Structure
Volume	535
Issue number	2
DOIs	https://doi.org/10.1016/0005-2795(78)90094-6
State	Published - Aug 21 1978

ASJC Scopus subject areas

General Medicine

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title = "Cleavage of a specific bond in troponin C by thrombin",

abstract = "Limited proteolysis of rabbit skeletal troponin C with bovine thrombin yielded two fragments, TH1 (Mr = 11 000) containing Ca2+ binding regions I-III and TH2 (Mr = 6000) containing region IV. Determination of the partial sequences of the fragments established the site of cleavage at Arg120-Ala121. Secondary cleavage by thrombin at other arginyl or lysyl residues in troponin C was ruled out by the sequence data and by the amino acid compositions of the two fragments.",

author = "Leavis, {P. C.} and S. Rosenfeld and Lu, {R. C.}",

note = "Funding Information: This work was supportedb y grantsf rom N.I.H. (HL-5949, AG00262),t he National Science Foundation (PCM-12171) and the Muscular Dystrophy Associationso f America,I nc. The authorsw ish to thank Dr. Frank Mannuzza of Miles Laboratoriesfo r a gift of purified thrombin and for helpful discussions.",

year = "1978",

month = aug,

day = "21",

doi = "10.1016/0005-2795(78)90094-6",

language = "English (US)",

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TY - JOUR

T1 - Cleavage of a specific bond in troponin C by thrombin

AU - Leavis, P. C.

AU - Rosenfeld, S.

AU - Lu, R. C.

N1 - Funding Information: This work was supportedb y grantsf rom N.I.H. (HL-5949, AG00262),t he National Science Foundation (PCM-12171) and the Muscular Dystrophy Associationso f America,I nc. The authorsw ish to thank Dr. Frank Mannuzza of Miles Laboratoriesfo r a gift of purified thrombin and for helpful discussions.

PY - 1978/8/21

Y1 - 1978/8/21

N2 - Limited proteolysis of rabbit skeletal troponin C with bovine thrombin yielded two fragments, TH1 (Mr = 11 000) containing Ca2+ binding regions I-III and TH2 (Mr = 6000) containing region IV. Determination of the partial sequences of the fragments established the site of cleavage at Arg120-Ala121. Secondary cleavage by thrombin at other arginyl or lysyl residues in troponin C was ruled out by the sequence data and by the amino acid compositions of the two fragments.

AB - Limited proteolysis of rabbit skeletal troponin C with bovine thrombin yielded two fragments, TH1 (Mr = 11 000) containing Ca2+ binding regions I-III and TH2 (Mr = 6000) containing region IV. Determination of the partial sequences of the fragments established the site of cleavage at Arg120-Ala121. Secondary cleavage by thrombin at other arginyl or lysyl residues in troponin C was ruled out by the sequence data and by the amino acid compositions of the two fragments.

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U2 - 10.1016/0005-2795(78)90094-6

DO - 10.1016/0005-2795(78)90094-6

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JO - BBA - Protein Structure

JF - BBA - Protein Structure

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ER -

Cleavage of a specific bond in troponin C by thrombin

Abstract

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