Cleavage of a specific bond in troponin C by thrombin

P. C. Leavis, Steven Rosenfeld, R. C. Lu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Limited proteolysis of rabbit skeletal troponin C with bovine thrombin yielded two fragments, TH1 (Mr = 11 000) containing Ca2+ binding regions I-III and TH2 (Mr = 6000) containing region IV. Determination of the partial sequences of the fragments established the site of cleavage at Arg120-Ala121. Secondary cleavage by thrombin at other arginyl or lysyl residues in troponin C was ruled out by the sequence data and by the amino acid compositions of the two fragments.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalBBA - Protein Structure
Volume535
Issue number2
DOIs
StatePublished - Aug 21 1978
Externally publishedYes

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Troponin C
Thrombin
Proteolysis
Sequence Analysis
Amino Acid Sequence
Rabbits
Amino Acids
Chemical analysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cleavage of a specific bond in troponin C by thrombin. / Leavis, P. C.; Rosenfeld, Steven; Lu, R. C.

In: BBA - Protein Structure, Vol. 535, No. 2, 21.08.1978, p. 281-286.

Research output: Contribution to journalArticle

Leavis, P. C. ; Rosenfeld, Steven ; Lu, R. C. / Cleavage of a specific bond in troponin C by thrombin. In: BBA - Protein Structure. 1978 ; Vol. 535, No. 2. pp. 281-286.
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