Circulating osteogenic precursor cells in heterotopic bone formation

Robin K. Suda, Paul C. Billings, Kevin P. Egan, Jung Hoon Kim, Ruth McCarrick-Walmsley, David L. Glaser, David L. Porter, Eileen M. Shore, Robert Pignolo

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Cells with osteogenic potential can be found in a variety of tissues. Here we show that circulating osteogenic precursor (COP) cells, a bone marrow-derived type I collagen+/CD45+ subpopulation of mononuclear adherent cells, are present in early preosseous fibroproliferative lesions in patients with fibrodysplasia ossificans progressiva (FOP) and nucleate heterotopic ossification (HO) in a murine in vivo implantation assay. Blood samples from patients with FOP with active episodes of HO contain significantly higher numbers of clonally derived COP cell colonies than patients with stable disease or unaffected individuals. The highest level of COP cells was found in a patient just before the clinical onset of an HO exacerbation. Our studies show that even COP cells derived from an unaffected individual can contribute to HO in genetically susceptible host tissue. The possibility that circulating, hematopoietic-derived cells with osteogenic potential can seed inflammatory sites has tremendous implications and, to our knowledge, represents the first example of their involvement in clinical HO. Thus, bone formation is not limited to cells of the mesenchymal lineage, and circulating cells of hematopoietic origin can also serve as osteogenic precursors at remote sites of tissue inflammation.

Original languageEnglish (US)
Pages (from-to)2209-2219
Number of pages11
JournalStem Cells
Volume27
Issue number9
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

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Osteogenesis
Heterotopic Ossification
Myositis Ossificans
Cell Lineage
Collagen Type I
Bone Marrow Cells
Seeds
Inflammation

Keywords

  • Bone marrow transplantation
  • Fibrodysplasia ossificans progressive
  • Heterotopic ossification
  • Mesenchymal progenitor cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Suda, R. K., Billings, P. C., Egan, K. P., Kim, J. H., McCarrick-Walmsley, R., Glaser, D. L., ... Pignolo, R. (2009). Circulating osteogenic precursor cells in heterotopic bone formation. Stem Cells, 27(9), 2209-2219. https://doi.org/10.1002/stem.150

Circulating osteogenic precursor cells in heterotopic bone formation. / Suda, Robin K.; Billings, Paul C.; Egan, Kevin P.; Kim, Jung Hoon; McCarrick-Walmsley, Ruth; Glaser, David L.; Porter, David L.; Shore, Eileen M.; Pignolo, Robert.

In: Stem Cells, Vol. 27, No. 9, 01.09.2009, p. 2209-2219.

Research output: Contribution to journalArticle

Suda, RK, Billings, PC, Egan, KP, Kim, JH, McCarrick-Walmsley, R, Glaser, DL, Porter, DL, Shore, EM & Pignolo, R 2009, 'Circulating osteogenic precursor cells in heterotopic bone formation', Stem Cells, vol. 27, no. 9, pp. 2209-2219. https://doi.org/10.1002/stem.150
Suda RK, Billings PC, Egan KP, Kim JH, McCarrick-Walmsley R, Glaser DL et al. Circulating osteogenic precursor cells in heterotopic bone formation. Stem Cells. 2009 Sep 1;27(9):2209-2219. https://doi.org/10.1002/stem.150
Suda, Robin K. ; Billings, Paul C. ; Egan, Kevin P. ; Kim, Jung Hoon ; McCarrick-Walmsley, Ruth ; Glaser, David L. ; Porter, David L. ; Shore, Eileen M. ; Pignolo, Robert. / Circulating osteogenic precursor cells in heterotopic bone formation. In: Stem Cells. 2009 ; Vol. 27, No. 9. pp. 2209-2219.
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