TY - JOUR
T1 - Circulating giant macrophages as a potential biomarker of solid tumors
AU - Adams, Daniel L.
AU - Martin, Stuart S.
AU - Alpaugh, R. Katherine
AU - Charpentier, Monica
AU - Tsai, Susan
AU - Bergan, Raymond C.
AU - Ogden, Irene M.
AU - Catalona, William
AU - Chumsri, Saranya
AU - Tang, Cha Mei
AU - Cristofanilli, Massimo
PY - 2014/3/4
Y1 - 2014/3/4
N2 - Tumor-associated macrophages (TAMs) derived from primary tumors are believed to facilitate circulating tumor cell (CTC) seeding of distant metastases, but the mechanisms of these processes are poorly understood. Although many studies have focused on the migration of CTCs, less attention has been given to TAMs that, like CTCs, derive from tumor sites. Using precision microfilters under low-flow conditions, we isolated circulating cancer-associated macrophage-like cells (CAMLs) from the peripheral blood of patients with breast, pancreatic, or prostate cancer. CAMLs, which are not found in healthy individuals, were found to express epithelial, monocytic, and endothelial protein markers and were observed bound to CTCs in circulation. These data support the hypothesis that disseminated TAMs can be used as a biomarker of advanced disease and suggest that they have a participatory role in tumor cell migration.
AB - Tumor-associated macrophages (TAMs) derived from primary tumors are believed to facilitate circulating tumor cell (CTC) seeding of distant metastases, but the mechanisms of these processes are poorly understood. Although many studies have focused on the migration of CTCs, less attention has been given to TAMs that, like CTCs, derive from tumor sites. Using precision microfilters under low-flow conditions, we isolated circulating cancer-associated macrophage-like cells (CAMLs) from the peripheral blood of patients with breast, pancreatic, or prostate cancer. CAMLs, which are not found in healthy individuals, were found to express epithelial, monocytic, and endothelial protein markers and were observed bound to CTCs in circulation. These data support the hypothesis that disseminated TAMs can be used as a biomarker of advanced disease and suggest that they have a participatory role in tumor cell migration.
KW - Blood cell biomarkers
KW - Cancer biomarker
KW - Cancer metastasis
KW - Cancer screening
KW - Liquid biopsy
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UR - http://www.scopus.com/inward/citedby.url?scp=84895802375&partnerID=8YFLogxK
U2 - 10.1073/pnas.1320198111
DO - 10.1073/pnas.1320198111
M3 - Article
C2 - 24550495
AN - SCOPUS:84895802375
SN - 0027-8424
VL - 111
SP - 3514
EP - 3519
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -