Circulating and renal vein levels of microRNAs in patients with renal artery stenosis

Moo Yong Park, Sandra M. Herrmann, Ahmed Saad, Robert Jay Widmer, Hui Tang, Xiang Yang Zhu, Amir Lerman, Stephen C. Textor, Lilach O. Lerman

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Background MicroRNAs (miRs) are small non-coding RNAs that are important regulators of gene expression and have been implicated in atherosclerosis. Kidney injury distal to atherosclerotic renal artery stenosis (ARAS) is aggravated by atherosclerosis. Therefore, this study tested the hypothesis that renal miR expression would be altered in patients with ARAS. Methods Patients with essential hypertension (EH; n = 13) or ARAS (n = 13) underwent a 3-day protocol study under controlled conditions. For miR levels, blood samples were collected from EH and ARAS renal vein (RV) and inferior vena cava or peripheral vein of matched normotensive healthy volunteers (HV; n = 13) and patients with coronary atherosclerosis (CA; n = 11). Single-renal blood flow was measured in EH and ARAS using computer tomography to calculate renal gradients and release of miRs. Results Glomerular filtration rate (GFR) was lower in ARAS compared with the other groups. Systemic levels of most miRs were elevated in CA. RV miR levels were lower than systemic levels in both ARAS and EH. GFR-adjusted RV levels of miR-21, 155 and 210 were reduced only in ARAS patients compared with systemic levels in HV, although cross-kidney gradients were not different from EH. RV levels of miR-21, 126, 155 and 210 correlated with GFR. Conclusions Levels of atherosclerosis-related miR-21, 126, 155 and 210 are decreased in the stenotic-kidney vein of ARAS compared with EH patients, likely due to decreased GFR. Yet, these miRs might be implicated in modulating renal injury in ARAS, and their RV level may be a marker reflecting their renal expression.

Original languageEnglish (US)
Pages (from-to)480-490
Number of pages11
JournalNephrology Dialysis Transplantation
Volume30
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • atherosclerosis
  • microRNA
  • renal artery stenosis

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

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