Circadian phase shifts to neuropeptide Y in vitro: Cellular communication and signal transduction

Stephany M. Biello, Diego A. Golombek, Kathryn M. Schak, Mary E. Harrington

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Mammalian circadian rhythms originate in the hypothalamic suprachiasmatic nuclei (SCN), from which rhythmic neural activity can be recorded in vitro. Application of neurochemicals can reset this rhythm. Here we determine cellular correlates of the phase-shifting properties of neuropeptide Y (NPY) on the hamster circadian clock in vitro. Drug or control treatments were applied to hypothalamic slices containing the SCN on the first day in vitro. The firing rates of individual cells were sampled on the second day in vitro. Control slices exhibited a peak in firing rate in the middle of the day. Microdrop application of NPY to the SCN phase advanced the time of peak firing rate. This phase-shifting effect of NPY was not altered by block of sodium channels with tetrodotoxin or block of calcium channels with cadmium and nickel, consistent with a direct postsynaptic site of action. Pretreatment with the glutamate receptor antagonists (DL-2-amino-5- phosphonovaleric acid and 6-cyano-7-nitroquinoxaline-2,3-dione disodium) also did not alter phase shifts to NPY. Blocking GABA(A) receptors with bicuculline (Bic) had effects only at very high (millimolar) doses of Bic, whereas blocking GABA(B) receptors did not alter effects of NPY. Phase shifts to NPY were blocked by pretreatment with inhibitors of protein kinase C (PKC), suggesting that PKC activation may be necessary for these effects. Bathing the slice in low Ca2+/high Mg2+ can block phase shifts to NPY, possibly via a depolarizing action. A depolarizing high K+ bath can also block NPY phase shifts. The results are consistent with direct action of NPY on pacemaker neurons, mediated through a signal transduction pathway that depends on activation of PKC.

Original languageEnglish (US)
Pages (from-to)8468-8475
Number of pages8
JournalJournal of Neuroscience
Volume17
Issue number21
StatePublished - 1997
Externally publishedYes

Fingerprint

Neuropeptide Y
Signal Transduction
Communication
Suprachiasmatic Nucleus
Protein Kinase C
Bicuculline
GABA-B Receptors
6-Cyano-7-nitroquinoxaline-2,3-dione
2-Amino-5-phosphonovalerate
In Vitro Techniques
Excitatory Amino Acid Antagonists
Circadian Clocks
Sodium Channels
Drug and Narcotic Control
Tetrodotoxin
GABA-A Receptors
Calcium Channels
Circadian Rhythm
Nickel
Cadmium

Keywords

  • Calcium
  • Circadian
  • GABA
  • Glutamate
  • Hamster
  • Neuropeptide Y
  • Phase shift
  • PKC
  • Suprachiasmatic nucleus
  • TTX

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Biello, S. M., Golombek, D. A., Schak, K. M., & Harrington, M. E. (1997). Circadian phase shifts to neuropeptide Y in vitro: Cellular communication and signal transduction. Journal of Neuroscience, 17(21), 8468-8475.

Circadian phase shifts to neuropeptide Y in vitro : Cellular communication and signal transduction. / Biello, Stephany M.; Golombek, Diego A.; Schak, Kathryn M.; Harrington, Mary E.

In: Journal of Neuroscience, Vol. 17, No. 21, 1997, p. 8468-8475.

Research output: Contribution to journalArticle

Biello, SM, Golombek, DA, Schak, KM & Harrington, ME 1997, 'Circadian phase shifts to neuropeptide Y in vitro: Cellular communication and signal transduction', Journal of Neuroscience, vol. 17, no. 21, pp. 8468-8475.
Biello, Stephany M. ; Golombek, Diego A. ; Schak, Kathryn M. ; Harrington, Mary E. / Circadian phase shifts to neuropeptide Y in vitro : Cellular communication and signal transduction. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 21. pp. 8468-8475.
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AB - Mammalian circadian rhythms originate in the hypothalamic suprachiasmatic nuclei (SCN), from which rhythmic neural activity can be recorded in vitro. Application of neurochemicals can reset this rhythm. Here we determine cellular correlates of the phase-shifting properties of neuropeptide Y (NPY) on the hamster circadian clock in vitro. Drug or control treatments were applied to hypothalamic slices containing the SCN on the first day in vitro. The firing rates of individual cells were sampled on the second day in vitro. Control slices exhibited a peak in firing rate in the middle of the day. Microdrop application of NPY to the SCN phase advanced the time of peak firing rate. This phase-shifting effect of NPY was not altered by block of sodium channels with tetrodotoxin or block of calcium channels with cadmium and nickel, consistent with a direct postsynaptic site of action. Pretreatment with the glutamate receptor antagonists (DL-2-amino-5- phosphonovaleric acid and 6-cyano-7-nitroquinoxaline-2,3-dione disodium) also did not alter phase shifts to NPY. Blocking GABA(A) receptors with bicuculline (Bic) had effects only at very high (millimolar) doses of Bic, whereas blocking GABA(B) receptors did not alter effects of NPY. Phase shifts to NPY were blocked by pretreatment with inhibitors of protein kinase C (PKC), suggesting that PKC activation may be necessary for these effects. Bathing the slice in low Ca2+/high Mg2+ can block phase shifts to NPY, possibly via a depolarizing action. A depolarizing high K+ bath can also block NPY phase shifts. The results are consistent with direct action of NPY on pacemaker neurons, mediated through a signal transduction pathway that depends on activation of PKC.

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