Cigarette smoke-induced mitochondrial fragmentation and dysfunction in human airway smooth muscle

Bharathi Aravamudan, Alexander Kiel, Michelle Freeman, Philippe Delmotte, Michael Thompson, Robert Vassallo, Gary C Sieck, Christina M Pabelick, Y.s. Prakash

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The balance between mitochondrial fission and fusion is crucial for mitochondria to perform its normal cellular functions. We hypothesized that cigarette smoke (CS) disrupts this balance and enhances mitochondrial dysfunction in the airway. In nonasthmatic human airway smooth muscle (ASM) cells, CS extract (CSE) induced mitochondrial fragmentation and damages their networked morphology in a concentration-dependent fashion, via increased expression of mitochondrial fission protein dynamin-related protein 1 (Drp1) and decreased fusion protein mitofusin (Mfn) 2. CSE effects on Drp1 vs. Mfn2 and mitochondrial network morphology involved reactive oxygen species (ROS), activation of extracellular signal-regulated kinase (ERK), phosphatidylinositol 3-kinase (PI3K)/ protein kinase B (Akt), protein kinase C (PKC) and proteasome pathways, as well as transcriptional regulation via factors such as NF-κB and nuclear erythroid 2-related factor 2. Inhibiting Drp1 prevented CSE effects on mitochondrial networks and ROS generation, whereas blocking Mfn2 had the opposite, detrimental effect. In ASM from asmatic patients, mitochondria exhibited substantial morphological defects at baseline and showed increased Drp1 but decreased Mfn2 expression, with exacerbating effects of CSE. Overall, these results highlight the importance of mitochondrial networks and their regulation in the context of cellular changes induced by insults such as inflammation (as in asthma) or CS. Altered mitochondrial fission/fusion proteins have a further potential to influence parameters such as ROS and cell proliferation and apoptosis relevant to airway diseases.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume306
Issue number9
DOIs
StatePublished - May 1 2014

Fingerprint

Smoke
Tobacco Products
Dynamins
Smooth Muscle
Mitochondrial Dynamics
Reactive Oxygen Species
Proteins
Mitochondria
Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-akt
Mitochondrial Proteins
Extracellular Signal-Regulated MAP Kinases
Proteasome Endopeptidase Complex
Protein Kinase C
Smooth Muscle Myocytes
Asthma
Cell Proliferation
Apoptosis
Inflammation

Keywords

  • Asthma
  • Dynamin-related protein 1
  • Mitochondria
  • Mitofusin 2
  • Signaling

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

Cigarette smoke-induced mitochondrial fragmentation and dysfunction in human airway smooth muscle. / Aravamudan, Bharathi; Kiel, Alexander; Freeman, Michelle; Delmotte, Philippe; Thompson, Michael; Vassallo, Robert; Sieck, Gary C; Pabelick, Christina M; Prakash, Y.s.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 306, No. 9, 01.05.2014.

Research output: Contribution to journalArticle

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