Chronic Restraint via Tail Immobilization of Mice: Effects on Corticosterone Levels and Other Physiologic Indices of Stress

Tiffani D. Rogers, Naomi M Gades, Jeffrey D. Kearby, Carlos K. Virgous, James T. Dalton

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chronic venous cannulation in mice is an acceptable and useful technique for repeated blood sampling or continuous intravenous administration of substances, for which mild restraint of the animal may be necessary. Because chronic restraint has drawn considerable attention in the animal welfare community, the purpose of this study was to evaluate physiologic indices of stress in mice restrained by using an established tail restraint method. Serum corticosterone levels and body, thymus, adrenal, and spleen weights on days 2, 5, 8, and 12 were compared between tail-restrained and unrestrained mice. There were no significant differences between the two groups at the time points evaluated. Corticosterone levels were highest on day 8 for both groups and were significantly different from those on days 2 (P<0.009) and 5 (P<0.04) for restrained mice and on day 2 (P<0.02) for unrestrained mice. Levels in both groups declined by day 12, suggesting habitation. Weight loss was observed in all mice whether restrained or unrestrained. Significant differences in body, thymus, adrenal, and spleen weights were not evident between restrained and unrestrained mice. This study provides important information for balancing issues of prolonged restraint, animal well-being, and research goals.

Original languageEnglish (US)
Pages (from-to)46-50
Number of pages5
JournalContemporary Topics in Laboratory Animal Science
Volume41
Issue number1
StatePublished - Jan 2002
Externally publishedYes

Fingerprint

corticosterone
Corticosterone
Immobilization
Tail
tail
mice
restraint of animals
Thymus Gland
spleen
Spleen
animal well-being
Weights and Measures
Animal Welfare
blood sampling
intravenous injection
Catheterization
Intravenous Administration
animal welfare
Weight Loss
weight loss

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Chronic Restraint via Tail Immobilization of Mice : Effects on Corticosterone Levels and Other Physiologic Indices of Stress. / Rogers, Tiffani D.; Gades, Naomi M; Kearby, Jeffrey D.; Virgous, Carlos K.; Dalton, James T.

In: Contemporary Topics in Laboratory Animal Science, Vol. 41, No. 1, 01.2002, p. 46-50.

Research output: Contribution to journalArticle

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