Chromosomal aneusomies detected by fluorescent in situ hybridization analysis in clinically localized prostate carcinoma

J. A. Brown, A. Alcaraz, S. Takahashi, D. L. Persons, M. M. Lieber, Robert Brian Jenkins

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Fluorescent in situ hybridization using 12 chromosome enumeration probes (for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 17, 18, X and Y) was used to evaluate fresh tumor touch preparations from 40 randomly selected radical prostatectomy specimens. Of the tumors 16 (40%) contained chromosomal aneusomies. Chromosome 8 was aneusomic in 9 tumors (23%). Gain of chromosome 7 was observed in 8 tumors (20%). Chromosome 17 was aneusomic in 4 cases, and chromosomes 10, 11, 12, 18 and Y were each aneusomic twice. Loss of chromosome 9 was observed in 1 tumor. Chromosomes 4, 6, and X were never aneusomic. The percentage of monosomy 17 nuclei was 2 to 4 times the amount noted with the other autosomes for tumor and benign tissue. Computer analysis demonstrated that these signals contained twice the signal density and were significantly different (p <0.0001) than the single diploid chromosome 17 signals. This result is consistent with homologous pairing of chromosome 17 in benign and neoplastic prostate tissue. Anomalies of chromosomes 8 and/or 7 were present in 14 of the 16 cases (88%) aneusomic by fluorescent in situ hybridization. High grade tumors were more likely to be aneuploid on fluorescent in situ hybridization (p <0.001). Tumors with chromosome 8 aneusomies were of higher stage (p <0.05). Fluorescent in situ hybridization is more sensitive than flow cytometry for the detection of aneusomy/aneuploidy. The prognostic relevance of these findings will require further investigation.

Original languageEnglish (US)
Pages (from-to)1157-1162
Number of pages6
JournalJournal of Urology
Volume152
Issue number4
StatePublished - 1994

Fingerprint

Fluorescence In Situ Hybridization
Prostate
Carcinoma
Chromosomes, Human, Pair 8
Chromosomes, Human, Pair 17
Neoplasms
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 7
Aneuploidy
Monosomy
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 11
Touch
Prostatectomy
Diploidy
Flow Cytometry

Keywords

  • chromosome abnormalities
  • genetic markers
  • hybridization
  • prostatic neoplasms

ASJC Scopus subject areas

  • Urology

Cite this

Chromosomal aneusomies detected by fluorescent in situ hybridization analysis in clinically localized prostate carcinoma. / Brown, J. A.; Alcaraz, A.; Takahashi, S.; Persons, D. L.; Lieber, M. M.; Jenkins, Robert Brian.

In: Journal of Urology, Vol. 152, No. 4, 1994, p. 1157-1162.

Research output: Contribution to journalArticle

Brown, JA, Alcaraz, A, Takahashi, S, Persons, DL, Lieber, MM & Jenkins, RB 1994, 'Chromosomal aneusomies detected by fluorescent in situ hybridization analysis in clinically localized prostate carcinoma', Journal of Urology, vol. 152, no. 4, pp. 1157-1162.
Brown, J. A. ; Alcaraz, A. ; Takahashi, S. ; Persons, D. L. ; Lieber, M. M. ; Jenkins, Robert Brian. / Chromosomal aneusomies detected by fluorescent in situ hybridization analysis in clinically localized prostate carcinoma. In: Journal of Urology. 1994 ; Vol. 152, No. 4. pp. 1157-1162.
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abstract = "Fluorescent in situ hybridization using 12 chromosome enumeration probes (for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 17, 18, X and Y) was used to evaluate fresh tumor touch preparations from 40 randomly selected radical prostatectomy specimens. Of the tumors 16 (40{\%}) contained chromosomal aneusomies. Chromosome 8 was aneusomic in 9 tumors (23{\%}). Gain of chromosome 7 was observed in 8 tumors (20{\%}). Chromosome 17 was aneusomic in 4 cases, and chromosomes 10, 11, 12, 18 and Y were each aneusomic twice. Loss of chromosome 9 was observed in 1 tumor. Chromosomes 4, 6, and X were never aneusomic. The percentage of monosomy 17 nuclei was 2 to 4 times the amount noted with the other autosomes for tumor and benign tissue. Computer analysis demonstrated that these signals contained twice the signal density and were significantly different (p <0.0001) than the single diploid chromosome 17 signals. This result is consistent with homologous pairing of chromosome 17 in benign and neoplastic prostate tissue. Anomalies of chromosomes 8 and/or 7 were present in 14 of the 16 cases (88{\%}) aneusomic by fluorescent in situ hybridization. High grade tumors were more likely to be aneuploid on fluorescent in situ hybridization (p <0.001). Tumors with chromosome 8 aneusomies were of higher stage (p <0.05). Fluorescent in situ hybridization is more sensitive than flow cytometry for the detection of aneusomy/aneuploidy. The prognostic relevance of these findings will require further investigation.",
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