Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation

M. J. Worsham, S. R. Wolman, T. E. Carey, R. J. Zarbo, M. S. Benninger, D. L. Van Dyke

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aims-Chromosomal aberrations in tumour cells are often not discernable by direct analysis. Although cell culture allows qualitative analysis of the karyotype, potential selection and evolution during growth in vitro may yield misleading data. To determine whether aberrations observed in vitro are representative of the original lesion, chromosomal aberrations found after prolonged growth in vitro of two squamous cell carcinomas of the head and neck (SSCHN) were evaluated with fluorescence in situ hybridisation (FISH) on the original tumour nuclei. Methods-Specific karyotypic aberrations identified in cultures of two squamous cell carcinomas were targets for FISH analysis on tumour sections. Chromosome painting mixtures were selected based on in vitro karyotypic data. FISH was performed on cultured interphase and metaphase cells, and on histological sections from the original tumours. Results-The 9cen and 17cen probes yielded FISH signals consistent with the aneusomies predicted for the respective chromosomes from the culture karyotypes. Whole chromosome 9 paint confirmed the prior existence in the turnouts of i(9p) and i(9q), although only the latter hybridised with the 9cen probe. FISH data also supported in vivo representation of the diploid and tetraploid tumour subclones observed in cultures. In tumour HFH-SCC-8a, FISH results were generally concordant between cultured interphase and metaphase cells and the histological sections, and improved the interpretation of marker chromosomes identified in culture. Conclusion-The karyotypes obtained in these cases after prolonged passage in culture were consistent with the genetic alterations in the original tumours.

Original languageEnglish (US)
Pages (from-to)42-46
Number of pages5
JournalJournal of Clinical Pathology - Molecular Pathology
Volume52
Issue number1
StatePublished - 1999
Externally publishedYes

Fingerprint

Fluorescence In Situ Hybridization
Chromosome Aberrations
Squamous Cell Carcinoma
Karyotype
Neoplasms
Interphase
Metaphase
Chromosome Painting
Chromosomes, Human, Pair 9
Paint
Tetraploidy
Growth
Diploidy
Genetic Markers
Cell Culture Techniques
Chromosomes
In Vitro Techniques

Keywords

  • Cell culture
  • Fluorescence in situ hybridisation
  • Karyotypic aberrations
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Worsham, M. J., Wolman, S. R., Carey, T. E., Zarbo, R. J., Benninger, M. S., & Van Dyke, D. L. (1999). Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation. Journal of Clinical Pathology - Molecular Pathology, 52(1), 42-46.

Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation. / Worsham, M. J.; Wolman, S. R.; Carey, T. E.; Zarbo, R. J.; Benninger, M. S.; Van Dyke, D. L.

In: Journal of Clinical Pathology - Molecular Pathology, Vol. 52, No. 1, 1999, p. 42-46.

Research output: Contribution to journalArticle

Worsham, MJ, Wolman, SR, Carey, TE, Zarbo, RJ, Benninger, MS & Van Dyke, DL 1999, 'Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation', Journal of Clinical Pathology - Molecular Pathology, vol. 52, no. 1, pp. 42-46.
Worsham, M. J. ; Wolman, S. R. ; Carey, T. E. ; Zarbo, R. J. ; Benninger, M. S. ; Van Dyke, D. L. / Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation. In: Journal of Clinical Pathology - Molecular Pathology. 1999 ; Vol. 52, No. 1. pp. 42-46.
@article{26f0553fa48244e98e0102364bc84141,
title = "Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation",
abstract = "Aims-Chromosomal aberrations in tumour cells are often not discernable by direct analysis. Although cell culture allows qualitative analysis of the karyotype, potential selection and evolution during growth in vitro may yield misleading data. To determine whether aberrations observed in vitro are representative of the original lesion, chromosomal aberrations found after prolonged growth in vitro of two squamous cell carcinomas of the head and neck (SSCHN) were evaluated with fluorescence in situ hybridisation (FISH) on the original tumour nuclei. Methods-Specific karyotypic aberrations identified in cultures of two squamous cell carcinomas were targets for FISH analysis on tumour sections. Chromosome painting mixtures were selected based on in vitro karyotypic data. FISH was performed on cultured interphase and metaphase cells, and on histological sections from the original tumours. Results-The 9cen and 17cen probes yielded FISH signals consistent with the aneusomies predicted for the respective chromosomes from the culture karyotypes. Whole chromosome 9 paint confirmed the prior existence in the turnouts of i(9p) and i(9q), although only the latter hybridised with the 9cen probe. FISH data also supported in vivo representation of the diploid and tetraploid tumour subclones observed in cultures. In tumour HFH-SCC-8a, FISH results were generally concordant between cultured interphase and metaphase cells and the histological sections, and improved the interpretation of marker chromosomes identified in culture. Conclusion-The karyotypes obtained in these cases after prolonged passage in culture were consistent with the genetic alterations in the original tumours.",
keywords = "Cell culture, Fluorescence in situ hybridisation, Karyotypic aberrations, Squamous cell carcinoma",
author = "Worsham, {M. J.} and Wolman, {S. R.} and Carey, {T. E.} and Zarbo, {R. J.} and Benninger, {M. S.} and {Van Dyke}, {D. L.}",
year = "1999",
language = "English (US)",
volume = "52",
pages = "42--46",
journal = "Molecular pathology : MP",
issn = "0021-9746",
publisher = "BMJ Publishing Group",
number = "1",

}

TY - JOUR

T1 - Chromosomal aberrations identified in culture of squamous carcinomas are confirmed by fluorescence in situ hybridisation

AU - Worsham, M. J.

AU - Wolman, S. R.

AU - Carey, T. E.

AU - Zarbo, R. J.

AU - Benninger, M. S.

AU - Van Dyke, D. L.

PY - 1999

Y1 - 1999

N2 - Aims-Chromosomal aberrations in tumour cells are often not discernable by direct analysis. Although cell culture allows qualitative analysis of the karyotype, potential selection and evolution during growth in vitro may yield misleading data. To determine whether aberrations observed in vitro are representative of the original lesion, chromosomal aberrations found after prolonged growth in vitro of two squamous cell carcinomas of the head and neck (SSCHN) were evaluated with fluorescence in situ hybridisation (FISH) on the original tumour nuclei. Methods-Specific karyotypic aberrations identified in cultures of two squamous cell carcinomas were targets for FISH analysis on tumour sections. Chromosome painting mixtures were selected based on in vitro karyotypic data. FISH was performed on cultured interphase and metaphase cells, and on histological sections from the original tumours. Results-The 9cen and 17cen probes yielded FISH signals consistent with the aneusomies predicted for the respective chromosomes from the culture karyotypes. Whole chromosome 9 paint confirmed the prior existence in the turnouts of i(9p) and i(9q), although only the latter hybridised with the 9cen probe. FISH data also supported in vivo representation of the diploid and tetraploid tumour subclones observed in cultures. In tumour HFH-SCC-8a, FISH results were generally concordant between cultured interphase and metaphase cells and the histological sections, and improved the interpretation of marker chromosomes identified in culture. Conclusion-The karyotypes obtained in these cases after prolonged passage in culture were consistent with the genetic alterations in the original tumours.

AB - Aims-Chromosomal aberrations in tumour cells are often not discernable by direct analysis. Although cell culture allows qualitative analysis of the karyotype, potential selection and evolution during growth in vitro may yield misleading data. To determine whether aberrations observed in vitro are representative of the original lesion, chromosomal aberrations found after prolonged growth in vitro of two squamous cell carcinomas of the head and neck (SSCHN) were evaluated with fluorescence in situ hybridisation (FISH) on the original tumour nuclei. Methods-Specific karyotypic aberrations identified in cultures of two squamous cell carcinomas were targets for FISH analysis on tumour sections. Chromosome painting mixtures were selected based on in vitro karyotypic data. FISH was performed on cultured interphase and metaphase cells, and on histological sections from the original tumours. Results-The 9cen and 17cen probes yielded FISH signals consistent with the aneusomies predicted for the respective chromosomes from the culture karyotypes. Whole chromosome 9 paint confirmed the prior existence in the turnouts of i(9p) and i(9q), although only the latter hybridised with the 9cen probe. FISH data also supported in vivo representation of the diploid and tetraploid tumour subclones observed in cultures. In tumour HFH-SCC-8a, FISH results were generally concordant between cultured interphase and metaphase cells and the histological sections, and improved the interpretation of marker chromosomes identified in culture. Conclusion-The karyotypes obtained in these cases after prolonged passage in culture were consistent with the genetic alterations in the original tumours.

KW - Cell culture

KW - Fluorescence in situ hybridisation

KW - Karyotypic aberrations

KW - Squamous cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=0032921623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032921623&partnerID=8YFLogxK

M3 - Article

VL - 52

SP - 42

EP - 46

JO - Molecular pathology : MP

JF - Molecular pathology : MP

SN - 0021-9746

IS - 1

ER -