Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair

Hui Zhou, Benjamin J. Madden, David C. Muddiman, Zhiguo Zhang

Research output: Contribution to journalArticle

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Abstract

In eukaryotic cells, chromatin is classified into euchromatin, which is active in transcription, and heterochromatin that silences transcription. Histones in these two domains contain distinct modifications. Chromatin assembly factor 1 (CAF-1) is a highly conserved protein that functions in DNA replication, DNA repair, and heterochromatin silencing. CAF-1 binds histones H3 and H4 and deposits histones onto DNA to form nucleosomes. However, modifications on H3 and H4 associated with CAF-1 are not known. Here, we have purified a complex containing CAF-1 and H3 and H4 from yeast cells and determined the modifications present on these histones using linear ion trap FT-ICR mass spectrometry. H4 that copurified with CAF-1 was a mixture of isoforms acetylated at lysines 5, 8, 12, and 16, whereas an H3 peptide methylated at lysine 79 and an H3 peptide acetylated at lysine 56 were detected. In yeast cell extracts, these two H3 modifications peaked in the late S phase with different kinetics. Moreover, the association of CAF-1 with H3 methylated at lysine 79 appeared to occur in the late S phase. Finally, cells lacking both Dot1p, the methyltransferase that methylates H3 lysine 79, and Cac1p, the large subunit of CAF-1, exhibited a dramatic loss of telomeric silencing and increased sensitivity to DNA damaging agents. Together, these data indicate that CAF-1 interacts with H3 methylated at lysine 79 during the processes of epigenetic silencing and DNA repair.

Original languageEnglish (US)
Pages (from-to)2852-2861
Number of pages10
JournalBiochemistry
Volume45
Issue number9
DOIs
StatePublished - Mar 7 2006

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Chromatin Assembly Factor-1
Genetic Epigenesis
DNA Repair
Histones
Lysine
Repair
DNA
Heterochromatin
Transcription
S Phase
Yeast
Yeasts
Cells
Euchromatin
Peptides
Nucleosomes
Methyltransferases
Eukaryotic Cells
Cell Extracts
DNA Replication

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. / Zhou, Hui; Madden, Benjamin J.; Muddiman, David C.; Zhang, Zhiguo.

In: Biochemistry, Vol. 45, No. 9, 07.03.2006, p. 2852-2861.

Research output: Contribution to journalArticle

Zhou, Hui ; Madden, Benjamin J. ; Muddiman, David C. ; Zhang, Zhiguo. / Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. In: Biochemistry. 2006 ; Vol. 45, No. 9. pp. 2852-2861.
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