Chondrocyte activation by interleukin-1: Synergism with fibroblast growth factor and phorbol myristate acetate

G. Bandara, C. W. Lin, H. I. Georgescu, D. Mendelow, Christopher H Evans

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Exposure to synovial factors or purified interleukin-1 (IL-1) induces the production of prostaglandin E2 (PGE2) and the neutral proteinases (NP) collagenase, gelatinase and stromelysin by lapine articular chondrocytes. Having frequently found our partially purified synovial preparations to elicit this process of chondrocyte activation more strongly than recombinant IL-1, Phadke's report of synergism between IL-1 and fibroblast growth factor (FGF) intrigued us. In our hands, basic FGF (1 ng/ml-1 μg/ml) did not activate chondrocytes but, in a dose-dependent manner, enhanced the production of PGE2 and NP by chondrocytes exposed to IL-1α or IL-1β (1-10 U/ml). Further examination determined that the basic FGF was a better synergist than acidic FGF. In view of reports that FGF activates protein kinase C, we tested whether phorbol myristate acetate (PMA) could substitute for FGF as a synergist. Not only did it do so, but PMA alone (0.1 ng/ml-100 ng/ml), unlike FGF, provoked the production of PGE2 by chondrocytes. The Ca2+ ionophore A23187 could not substitute for FGF in enhancing induction of the NP. Using a cDNA probe, we confirmed that the synergistic effects of both FGF and PMA upon IL-1 mediated collagenase induction, were associated with an increased abundance of collagenase mRNA.

Original languageEnglish (US)
Pages (from-to)439-441
Number of pages3
JournalAgents and Actions
Volume27
Issue number3-4
StatePublished - 1989
Externally publishedYes

Fingerprint

Fibroblast Growth Factors
Tetradecanoylphorbol Acetate
Chondrocytes
Interleukin-1
Chemical activation
Collagenases
Dinoprostone
Fibroblast Growth Factor 1
Peptide Hydrolases
Fibroblast Growth Factor 2
Matrix Metalloproteinase 3
Gelatinases
Ionophores
Calcimycin
Protein Kinase C
Complementary DNA
Joints
Messenger RNA

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Toxicology

Cite this

Chondrocyte activation by interleukin-1 : Synergism with fibroblast growth factor and phorbol myristate acetate. / Bandara, G.; Lin, C. W.; Georgescu, H. I.; Mendelow, D.; Evans, Christopher H.

In: Agents and Actions, Vol. 27, No. 3-4, 1989, p. 439-441.

Research output: Contribution to journalArticle

Bandara, G. ; Lin, C. W. ; Georgescu, H. I. ; Mendelow, D. ; Evans, Christopher H. / Chondrocyte activation by interleukin-1 : Synergism with fibroblast growth factor and phorbol myristate acetate. In: Agents and Actions. 1989 ; Vol. 27, No. 3-4. pp. 439-441.
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