Chondrocyte activation by interleukin-1: Analysis of the synergistic properties of fibroblast growth factor and phorbol myristate acetate

G. Bandara, C. W. Lin, H. I. Georgescu, D. Mendelow, Christopher H Evans

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Following activation, monolayers of lapine articular chondrocytes secreted into their culture media large amounts of prostaglandin E2 (PGE2) and the neutral metalloproteinases collagenase and gelatinase. Partially purified preparations of synovial "chondrocyte activating factors" (CAF), which contain interleukin-1 (IL-1), generally proved stronger activators of chondrocytes than recombinant, human, IL-1α (rHIL-1α) or IL-1β (rHIL-1β). The presence of synergistic cytokines within the synovial material provides one possible explanation of this discrepancy. As first reported by K. Phadke (1987, Biochem. Biophys. Res. Commun.142, 448-453) fibroblast growth factor (FGF) synergized with rHIL-1 in promoting the synthesis of neutral metalloproteinases. In our hands FGF alone did not induce neutral metalloproteinases and increased PGE2 synthesis only modestly. However, at doses from 1 ng/ml to 1 μg/ml, FGF progressively enhanced the synthesis of PGE2, collagenase, and gelatinase by chondrocytes responding to rHIL-1. Acidic and basic FGF synergized equally well with both rHIL-1α and rHIL-1β. Phorbol myristate acetate (PMA), but not the Ca2+-ionophore A23187, could substitute for FGF as a synergist. PMA alone was a poor inducer of collagenase or gelatinase but, unlike FGF, it greatly enhanced the synthesis of PGE2 by chondrocytes. Dot-blot analyses with a cDNA probe to collagenase mRNA confirmed that partially purified synovial CAF induced collagenase mRNA more effectively than rHIL-1, with rHIL-1α being superior to rHIL-1β in this regard. The synergistic effects of both FGF and PMA upon IL-1-mediated collagenase induction were associated with increased abundance of collagenase mRNA.

Original languageEnglish (US)
Pages (from-to)539-547
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume274
Issue number2
DOIs
StatePublished - Nov 1 1989
Externally publishedYes

Fingerprint

Fibroblast Growth Factors
Tetradecanoylphorbol Acetate
Chondrocytes
Interleukin-1
Chemical activation
Collagenases
Dinoprostone
Gelatinases
Metalloproteases
Messenger RNA
Fibroblast Growth Factor 1
Ionophores
Calcimycin
Fibroblast Growth Factor 2
Culture Media
Monolayers
Complementary DNA
Joints

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chondrocyte activation by interleukin-1 : Analysis of the synergistic properties of fibroblast growth factor and phorbol myristate acetate. / Bandara, G.; Lin, C. W.; Georgescu, H. I.; Mendelow, D.; Evans, Christopher H.

In: Archives of Biochemistry and Biophysics, Vol. 274, No. 2, 01.11.1989, p. 539-547.

Research output: Contribution to journalArticle

@article{50b724a95a1449bba35df9f90eca0ad2,
title = "Chondrocyte activation by interleukin-1: Analysis of the synergistic properties of fibroblast growth factor and phorbol myristate acetate",
abstract = "Following activation, monolayers of lapine articular chondrocytes secreted into their culture media large amounts of prostaglandin E2 (PGE2) and the neutral metalloproteinases collagenase and gelatinase. Partially purified preparations of synovial {"}chondrocyte activating factors{"} (CAF), which contain interleukin-1 (IL-1), generally proved stronger activators of chondrocytes than recombinant, human, IL-1α (rHIL-1α) or IL-1β (rHIL-1β). The presence of synergistic cytokines within the synovial material provides one possible explanation of this discrepancy. As first reported by K. Phadke (1987, Biochem. Biophys. Res. Commun.142, 448-453) fibroblast growth factor (FGF) synergized with rHIL-1 in promoting the synthesis of neutral metalloproteinases. In our hands FGF alone did not induce neutral metalloproteinases and increased PGE2 synthesis only modestly. However, at doses from 1 ng/ml to 1 μg/ml, FGF progressively enhanced the synthesis of PGE2, collagenase, and gelatinase by chondrocytes responding to rHIL-1. Acidic and basic FGF synergized equally well with both rHIL-1α and rHIL-1β. Phorbol myristate acetate (PMA), but not the Ca2+-ionophore A23187, could substitute for FGF as a synergist. PMA alone was a poor inducer of collagenase or gelatinase but, unlike FGF, it greatly enhanced the synthesis of PGE2 by chondrocytes. Dot-blot analyses with a cDNA probe to collagenase mRNA confirmed that partially purified synovial CAF induced collagenase mRNA more effectively than rHIL-1, with rHIL-1α being superior to rHIL-1β in this regard. The synergistic effects of both FGF and PMA upon IL-1-mediated collagenase induction were associated with increased abundance of collagenase mRNA.",
author = "G. Bandara and Lin, {C. W.} and Georgescu, {H. I.} and D. Mendelow and Evans, {Christopher H}",
year = "1989",
month = "11",
day = "1",
doi = "10.1016/0003-9861(89)90468-2",
language = "English (US)",
volume = "274",
pages = "539--547",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Chondrocyte activation by interleukin-1

T2 - Analysis of the synergistic properties of fibroblast growth factor and phorbol myristate acetate

AU - Bandara, G.

AU - Lin, C. W.

AU - Georgescu, H. I.

AU - Mendelow, D.

AU - Evans, Christopher H

PY - 1989/11/1

Y1 - 1989/11/1

N2 - Following activation, monolayers of lapine articular chondrocytes secreted into their culture media large amounts of prostaglandin E2 (PGE2) and the neutral metalloproteinases collagenase and gelatinase. Partially purified preparations of synovial "chondrocyte activating factors" (CAF), which contain interleukin-1 (IL-1), generally proved stronger activators of chondrocytes than recombinant, human, IL-1α (rHIL-1α) or IL-1β (rHIL-1β). The presence of synergistic cytokines within the synovial material provides one possible explanation of this discrepancy. As first reported by K. Phadke (1987, Biochem. Biophys. Res. Commun.142, 448-453) fibroblast growth factor (FGF) synergized with rHIL-1 in promoting the synthesis of neutral metalloproteinases. In our hands FGF alone did not induce neutral metalloproteinases and increased PGE2 synthesis only modestly. However, at doses from 1 ng/ml to 1 μg/ml, FGF progressively enhanced the synthesis of PGE2, collagenase, and gelatinase by chondrocytes responding to rHIL-1. Acidic and basic FGF synergized equally well with both rHIL-1α and rHIL-1β. Phorbol myristate acetate (PMA), but not the Ca2+-ionophore A23187, could substitute for FGF as a synergist. PMA alone was a poor inducer of collagenase or gelatinase but, unlike FGF, it greatly enhanced the synthesis of PGE2 by chondrocytes. Dot-blot analyses with a cDNA probe to collagenase mRNA confirmed that partially purified synovial CAF induced collagenase mRNA more effectively than rHIL-1, with rHIL-1α being superior to rHIL-1β in this regard. The synergistic effects of both FGF and PMA upon IL-1-mediated collagenase induction were associated with increased abundance of collagenase mRNA.

AB - Following activation, monolayers of lapine articular chondrocytes secreted into their culture media large amounts of prostaglandin E2 (PGE2) and the neutral metalloproteinases collagenase and gelatinase. Partially purified preparations of synovial "chondrocyte activating factors" (CAF), which contain interleukin-1 (IL-1), generally proved stronger activators of chondrocytes than recombinant, human, IL-1α (rHIL-1α) or IL-1β (rHIL-1β). The presence of synergistic cytokines within the synovial material provides one possible explanation of this discrepancy. As first reported by K. Phadke (1987, Biochem. Biophys. Res. Commun.142, 448-453) fibroblast growth factor (FGF) synergized with rHIL-1 in promoting the synthesis of neutral metalloproteinases. In our hands FGF alone did not induce neutral metalloproteinases and increased PGE2 synthesis only modestly. However, at doses from 1 ng/ml to 1 μg/ml, FGF progressively enhanced the synthesis of PGE2, collagenase, and gelatinase by chondrocytes responding to rHIL-1. Acidic and basic FGF synergized equally well with both rHIL-1α and rHIL-1β. Phorbol myristate acetate (PMA), but not the Ca2+-ionophore A23187, could substitute for FGF as a synergist. PMA alone was a poor inducer of collagenase or gelatinase but, unlike FGF, it greatly enhanced the synthesis of PGE2 by chondrocytes. Dot-blot analyses with a cDNA probe to collagenase mRNA confirmed that partially purified synovial CAF induced collagenase mRNA more effectively than rHIL-1, with rHIL-1α being superior to rHIL-1β in this regard. The synergistic effects of both FGF and PMA upon IL-1-mediated collagenase induction were associated with increased abundance of collagenase mRNA.

UR - http://www.scopus.com/inward/record.url?scp=0024470051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024470051&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(89)90468-2

DO - 10.1016/0003-9861(89)90468-2

M3 - Article

C2 - 2552926

AN - SCOPUS:0024470051

VL - 274

SP - 539

EP - 547

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -