Chondrocyte activation by a putative interleukin-1 derived from lapine polymorphonuclear leukocytes

S. Watanabe, H. I. Georgescu, D. B. Kuhns, Christopher H Evans

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Polymorphonuclear leukocytes (PMNs) recovered from 4-h lapine peritoneal exudates contained factors which provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E2 by lapine articular chondrocytes. Rapid secretion of these factors occurred after exposing the polymorphs to phorbol myristate acetate or formyl-MetLeu-Phe. Fractionation of polymorph lysates by HPLC size exclusion chromatography provided a molecular weight of approximately 14,000 for the active principle. Examination of the most active fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by silver staining, confirmed the presence of a single band with this apparent molecular weight. Isoelectric focusing of this fraction revealed the presence of four distinct bands with the pI values 6.90, 7.05, 7.45, and 7.55. This fraction tested positive in a bioassay for interleukin-1. We were unable to activate chondrocytes by exposure to extracts of human PMNs from either peripheral blood or inflammatory synovial fluid.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume270
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Chondrocytes
Polymorphism
Interleukin-1
Neutrophils
Molecular Weight
Chemical activation
Molecular weight
Gelatinases
Silver Staining
Size exclusion chromatography
Bioassay
Synovial Fluid
Isoelectric Focusing
Tetradecanoylphorbol Acetate
Collagenases
Exudates and Transudates
Fractionation
Electrophoresis
Silver
Dinoprostone

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chondrocyte activation by a putative interleukin-1 derived from lapine polymorphonuclear leukocytes. / Watanabe, S.; Georgescu, H. I.; Kuhns, D. B.; Evans, Christopher H.

In: Archives of Biochemistry and Biophysics, Vol. 270, No. 1, 1989, p. 69-76.

Research output: Contribution to journalArticle

@article{0b814e670a7143d9acc83a9fecd1f157,
title = "Chondrocyte activation by a putative interleukin-1 derived from lapine polymorphonuclear leukocytes",
abstract = "Polymorphonuclear leukocytes (PMNs) recovered from 4-h lapine peritoneal exudates contained factors which provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E2 by lapine articular chondrocytes. Rapid secretion of these factors occurred after exposing the polymorphs to phorbol myristate acetate or formyl-MetLeu-Phe. Fractionation of polymorph lysates by HPLC size exclusion chromatography provided a molecular weight of approximately 14,000 for the active principle. Examination of the most active fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by silver staining, confirmed the presence of a single band with this apparent molecular weight. Isoelectric focusing of this fraction revealed the presence of four distinct bands with the pI values 6.90, 7.05, 7.45, and 7.55. This fraction tested positive in a bioassay for interleukin-1. We were unable to activate chondrocytes by exposure to extracts of human PMNs from either peripheral blood or inflammatory synovial fluid.",
author = "S. Watanabe and Georgescu, {H. I.} and Kuhns, {D. B.} and Evans, {Christopher H}",
year = "1989",
doi = "10.1016/0003-9861(89)90008-8",
language = "English (US)",
volume = "270",
pages = "69--76",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Chondrocyte activation by a putative interleukin-1 derived from lapine polymorphonuclear leukocytes

AU - Watanabe, S.

AU - Georgescu, H. I.

AU - Kuhns, D. B.

AU - Evans, Christopher H

PY - 1989

Y1 - 1989

N2 - Polymorphonuclear leukocytes (PMNs) recovered from 4-h lapine peritoneal exudates contained factors which provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E2 by lapine articular chondrocytes. Rapid secretion of these factors occurred after exposing the polymorphs to phorbol myristate acetate or formyl-MetLeu-Phe. Fractionation of polymorph lysates by HPLC size exclusion chromatography provided a molecular weight of approximately 14,000 for the active principle. Examination of the most active fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by silver staining, confirmed the presence of a single band with this apparent molecular weight. Isoelectric focusing of this fraction revealed the presence of four distinct bands with the pI values 6.90, 7.05, 7.45, and 7.55. This fraction tested positive in a bioassay for interleukin-1. We were unable to activate chondrocytes by exposure to extracts of human PMNs from either peripheral blood or inflammatory synovial fluid.

AB - Polymorphonuclear leukocytes (PMNs) recovered from 4-h lapine peritoneal exudates contained factors which provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E2 by lapine articular chondrocytes. Rapid secretion of these factors occurred after exposing the polymorphs to phorbol myristate acetate or formyl-MetLeu-Phe. Fractionation of polymorph lysates by HPLC size exclusion chromatography provided a molecular weight of approximately 14,000 for the active principle. Examination of the most active fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by silver staining, confirmed the presence of a single band with this apparent molecular weight. Isoelectric focusing of this fraction revealed the presence of four distinct bands with the pI values 6.90, 7.05, 7.45, and 7.55. This fraction tested positive in a bioassay for interleukin-1. We were unable to activate chondrocytes by exposure to extracts of human PMNs from either peripheral blood or inflammatory synovial fluid.

UR - http://www.scopus.com/inward/record.url?scp=0024594261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024594261&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(89)90008-8

DO - 10.1016/0003-9861(89)90008-8

M3 - Article

VL - 270

SP - 69

EP - 76

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -