TY - JOUR
T1 - Choline cannot be replaced by propanolamine in mice
AU - Li, Zhaoyu
AU - Vance, Dennis E.
N1 - Funding Information:
We thank Sandra Ungarian, Susanne Lingrell, Audric Moses, and Priscilla Gao for excellent technical assistance. We thank Dr. Dennis Voelker for providing the phosphatidylpropanolamine standard. This research was supported by a grant from the Canadian Institutes of Health Research (MOP5182). D.E.V. is a Scientist of the Alberta Heritage Foundation for Medical Research and holds the Canada Research Chair in Molecular and Cell Biology of Lipids.
PY - 2007/4
Y1 - 2007/4
N2 - Choline is an important nutrient for humans and animals. Animals obtain choline from the diet and from the catabolism of phosphatidylcholine made by phosphatidylethanolamine N-methyltransferase (PEMT). The unique model of complete choline deprivation is Pemt-/- mice that are fed a choline-deficient diet. This model, therefore, can be used for the examination of choline substitutes in mammalian systems. Recently, propanolamine was found to be a replacement for choline in yeast. Thus, we tested to see whether or not choline can be replaced by propanolamine in mice. Mice were fed a choline-deficient diet and supplemented with either methionine, 2-amino-propanol, 2-amino-isopropanol and 3-amino-propanol. We were unable to detect the formation of any of the possible phosphatidylpropanolamines. Moreover, none of them prevented liver damage, reduction of hepatic phosphatidylcholine levels or fatty liver induced in choline-deficient-Pemt-/- mice. These results suggest that choline in mice cannot be replaced by any of the three propanolamine derivatives.
AB - Choline is an important nutrient for humans and animals. Animals obtain choline from the diet and from the catabolism of phosphatidylcholine made by phosphatidylethanolamine N-methyltransferase (PEMT). The unique model of complete choline deprivation is Pemt-/- mice that are fed a choline-deficient diet. This model, therefore, can be used for the examination of choline substitutes in mammalian systems. Recently, propanolamine was found to be a replacement for choline in yeast. Thus, we tested to see whether or not choline can be replaced by propanolamine in mice. Mice were fed a choline-deficient diet and supplemented with either methionine, 2-amino-propanol, 2-amino-isopropanol and 3-amino-propanol. We were unable to detect the formation of any of the possible phosphatidylpropanolamines. Moreover, none of them prevented liver damage, reduction of hepatic phosphatidylcholine levels or fatty liver induced in choline-deficient-Pemt-/- mice. These results suggest that choline in mice cannot be replaced by any of the three propanolamine derivatives.
KW - Choline
KW - Choline-deficient
KW - Phosphatidylcholine
KW - Phosphatidylethanolamine N-methyltransferase
KW - Propanolamine
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U2 - 10.1016/j.bbalip.2007.01.003
DO - 10.1016/j.bbalip.2007.01.003
M3 - Article
C2 - 17292664
AN - SCOPUS:34047227753
SN - 1388-1981
VL - 1771
SP - 486
EP - 490
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 4
ER -