Cholesterol sulfation in human liver: Catalysis by dehydroepiandrosterone sulfotransferase

I. A. Aksoy, D. M. Otterness, R. M. Weinshilboum

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Cholesterol can undergo sulfate conjugation to form cholesterol 3-sulfate. Our experiments were performed to determine whether human liver cytosol could catalyze the sulfation of cholesterol, and, if so, whether any of the three well-characterized human hepatic cytosolic sulfotransferases, dehydroepiandrosterone sulfotransferase (DHEA ST), thermostable (TS) phenol sulfotransferase (PST), or thermolabile (TL) PST might participate in the reaction. On the basis of substrate kinetics, two 'forms' of cholesterol sulfotransferase (CST) activity were present in human liver cytosol, one with high and one with low affinity for cholesterol. Apparent K(M) values of the high- and low-affinity activities were 0.14 and 15 μM for cholesterol and 0.30 and 0.19 μM for 3'-phosphoadenosine-5'-phosphosulfate, respectively. Both kinetic forms of CST activity had thermal inactivation profiles similar to those of DHEA ST and TS PST, but both were more thermostable than was TL PST. Enzyme inhibition studies performed with 2,6-dichloro-4-nitrophenol (DCNP) showed that inhibition profiles for both high- and low-affinity CST activities were similar to those of DHEA ST and TL PST, but both were more resistant to DCNP inhibition than was TS PST. Experiments performed with 20 individual human liver samples confirmed these observations and demonstrated highly significant correlations between both high- and low-affinity CST activities and DHEA ST activity (r(s) = 0.740, p = 0.0001 and r(s) = 0.767, p < 0.0001, respectively). However, the level of activity of neither kinetic form of CST activity was significantly correlated with either TS or TL PST activities. Finally, COS-1 cells transfected with human liver DHEA ST cDNA expressed both high- and low-affinity CST activities with biphasic substrate kinetics for cholesterol similar to those found in human hepatic cytosol. All of these results were compatible with the conclusion that the sulfation of cholesterol can occur in human liver cytosol and that DHEA ST is the major enzyme that catalyzes that reaction.

Original languageEnglish (US)
Pages (from-to)268-276
Number of pages9
JournalDrug Metabolism and Disposition
Volume21
Issue number2
StatePublished - Jan 1 1993

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ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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