Choice of infusion-sampling mode for tracer studies of free fatty acid metabolism

M. D. Jensen, P. J. Rogers, M. G. Ellman, J. M. Miles

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

To determine the preferred infusion-sampling mode for isotopic studies of free fatty acid (FFA) metabolism, tracer ([14C]palmitate) was infused into the left ventricle of five anesthetized dogs, and tracee ([3H]palmitate) was infused into three separate peripheral veins of each dog. The [14C]palmitate specific activity (SA) was lower in mixed venous than arterial blood, and [3H]palmitate SA was equal in both sites. The actual infusion rate of [3H]palmitate [2.15 ± 0.31 x 105 disintegrations/min (dpm) · kg-1 · min-1] could be accurately predicted (2.14 ± 0.32 x 105 dpm · kg-1 · min-1) using the known [14C]palmitate infusion rate and the arterial plasma [14C]-to-[3H]palmitate ratio. In contrast, the mixed venous [14C]-to-[3H]palmitate ratio resulted in overestimates (P < 0.05) of the actual [3H]palmitate infusion rate. In summary, venous tracer infusion with arterial blood sampling for FFA tracer studies provides the most accurate estimates of tracee rate of appearance.

Original languageEnglish (US)
Pages (from-to)17/5
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume254
Issue number5
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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