Chimeric measles viruses with a foreign envelope

Pius Spielhofer, Thomas Bächi, Thomas Fehr, Gudrun Christiansen, Roberto Cattaneo, Karin Kaelin, Martin A. Billeter, Hussein Y. Naim

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Measles virus (MV) and vesicular stomatitis virus (VSV) are both members of the Mononegavirales but are only distantly related. We generated two genetically stable chimeric viruses. In MGV, the reading frames of the MV envelope glycoproteins H and F were substituted by a single reading frame encoding the VSV G glycoprotein; MG/FV is similar but encodes a G/F hybrid in which the VSV G cytoplasmic tail was replaced by that of MV F. In contrast to MG/FV, MGV virions do not contain the MV matrix (M) protein. This demonstrates that virus assembly is possible in the absence of M; conversely, the cytoplasmic domain of F allows incorporation of M and enhances assembly. The formation of chimeric viruses was substantially delayed and the titers obtained were reduced about 50-fold in comparison to standard MV. In the novel chimeras, transcription and replication are mediated by the MV ribonucleoproteins but the envelope glycoproteins dictate the host range. Mice immunized with the chimeric viruses were protected against lethal doses of wild-type VSV. These findings suggest that it is feasible to construct MV variants bearing a variety of different envelopes for use as vaccines or for gene therapeutic purposes.

Original languageEnglish (US)
Pages (from-to)2150-2159
Number of pages10
JournalJournal of Virology
Volume72
Issue number3
StatePublished - Mar 1998
Externally publishedYes

Fingerprint

Measles virus
Vesiculovirus
Vesicular Stomatitis
Viruses
glycoproteins
Reading Frames
Glycoproteins
viruses
Mononegavirales
Virus Assembly
ribonucleoproteins
Ribonucleoproteins
chimerism
Host Specificity
lethal dose
virion
host range
Virion
Tail
tail

ASJC Scopus subject areas

  • Immunology

Cite this

Spielhofer, P., Bächi, T., Fehr, T., Christiansen, G., Cattaneo, R., Kaelin, K., ... Naim, H. Y. (1998). Chimeric measles viruses with a foreign envelope. Journal of Virology, 72(3), 2150-2159.

Chimeric measles viruses with a foreign envelope. / Spielhofer, Pius; Bächi, Thomas; Fehr, Thomas; Christiansen, Gudrun; Cattaneo, Roberto; Kaelin, Karin; Billeter, Martin A.; Naim, Hussein Y.

In: Journal of Virology, Vol. 72, No. 3, 03.1998, p. 2150-2159.

Research output: Contribution to journalArticle

Spielhofer, P, Bächi, T, Fehr, T, Christiansen, G, Cattaneo, R, Kaelin, K, Billeter, MA & Naim, HY 1998, 'Chimeric measles viruses with a foreign envelope', Journal of Virology, vol. 72, no. 3, pp. 2150-2159.
Spielhofer P, Bächi T, Fehr T, Christiansen G, Cattaneo R, Kaelin K et al. Chimeric measles viruses with a foreign envelope. Journal of Virology. 1998 Mar;72(3):2150-2159.
Spielhofer, Pius ; Bächi, Thomas ; Fehr, Thomas ; Christiansen, Gudrun ; Cattaneo, Roberto ; Kaelin, Karin ; Billeter, Martin A. ; Naim, Hussein Y. / Chimeric measles viruses with a foreign envelope. In: Journal of Virology. 1998 ; Vol. 72, No. 3. pp. 2150-2159.
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