Chemokine (C-X-C) ligand 12 facilitates trafficking of donor spermatogonial stem cells

Zhiyv Niu, Shaun M. Goodyear, Mary R. Avarbock, Ralph L. Brinster

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The chemokine (C-X-C) receptor type 4 (CXCR4) is an early marker of primordial germ cells (PGCs) essential for their migration and colonization of the gonads. In spermatogonial stem cells (SSCs), the expression of CXCR4 is promoted by the self-renewal factor, glial cell line-derived neurotrophic factor (GDNF). Here, we demonstrate an important role of CXCR4 during donor mouse SSCs reoccupation of the endogenous niche in recipient testis. Silencing of CXCR4 expression in mouse SSCs dramatically reduced the number of donor stem cell-derived colonies, whereas colony morphology and spermatogenesis were comparable to controls. Inhibition of CXCR4 signaling using a small molecule inhibitor (AMD3100) during the critical window of homing also significantly lowered the efficiency of donor-derived SSCs to establish spermatogenic colonies in recipient mice; however, the self-renewal of SSCs was not affected by exposure to AMD3100. Rather, in vitro migration assays demonstrate the influence of CXCR4-CXCL12 signaling in promoting germ cell migration. Together, these studies suggest that CXCR4-CXCL12 signaling functions to promote homing of SSCs towards the stem cell niche and plays a critical role in reestablishing spermatogenesis.

Original languageEnglish (US)
Article number5796305
JournalStem Cells International
Volume2016
DOIs
StatePublished - Jan 1 2016

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CXC Chemokines
Stem Cells
Ligands
Spermatogenesis
Germ Cells
Stem Cell Niche
Glial Cell Line-Derived Neurotrophic Factor
Gonads
Cell Movement
Testis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Chemokine (C-X-C) ligand 12 facilitates trafficking of donor spermatogonial stem cells. / Niu, Zhiyv; Goodyear, Shaun M.; Avarbock, Mary R.; Brinster, Ralph L.

In: Stem Cells International, Vol. 2016, 5796305, 01.01.2016.

Research output: Contribution to journalArticle

Niu, Zhiyv ; Goodyear, Shaun M. ; Avarbock, Mary R. ; Brinster, Ralph L. / Chemokine (C-X-C) ligand 12 facilitates trafficking of donor spermatogonial stem cells. In: Stem Cells International. 2016 ; Vol. 2016.
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