Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii

Edna S. Kaneshiro, Jayne E. Ellis, Zengkui Guo, Koka Jayasimhulu, J. Nicholas Maiorano, Kathryn A. Kallam

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pneumocystis carinii causes pneumonitis in immunodeficient individuals and is a prevalent opportunistic infection of patients with AIDS. This pathogen resides extracellularly in the hypophase lining the alveolar epithelium, which is highly enriched in lung surfactant lipids. Procedures yielding highly pure organism preparations that enable reliable biochemical analyses of organisms isolated from the lungs of infected laboratory animals have been developed. The results of the present study revealed that the fatty acid profiles of total lipids, the neutral lipid fraction, and individual neutral lipid classes of lungs from normal and immunosuppressed rats as well as P. carinii were grossly similar, although some quantitative differences were detected. One qualitative exception found was the detection in P. carinii of the rare fatty acid cis-9,10-epoxy stearic acid, which was not detected in the lipids of rat lungs. The detection of this fatty acid in P. carinii may also have important taxonomic implications. Unlike phospholipids, many of the fatty acids of nonmembrane neutral lipids may be utilized by P. carinii for other cellular functions, such as stored reserves for energy production and precursors for organism-specific membrane lipids. The present study represents the first report of detailed fatty acid analyses of individual neutral lipid classes of this important opportunistic pathogen.

Original languageEnglish (US)
Pages (from-to)4105-4114
Number of pages10
JournalInfection and Immunity
Volume64
Issue number10
StatePublished - Oct 2 1996
Externally publishedYes

Fingerprint

Pneumocystis carinii
Fatty Acids
Lipids
Lung
Opportunistic Infections
Laboratory Animals
Membrane Lipids
stearic acid
Surface-Active Agents
Phospholipids
Pneumonia
Acquired Immunodeficiency Syndrome
Epithelium

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Kaneshiro, E. S., Ellis, J. E., Guo, Z., Jayasimhulu, K., Nicholas Maiorano, J., & Kallam, K. A. (1996). Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii. Infection and Immunity, 64(10), 4105-4114.

Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii. / Kaneshiro, Edna S.; Ellis, Jayne E.; Guo, Zengkui; Jayasimhulu, Koka; Nicholas Maiorano, J.; Kallam, Kathryn A.

In: Infection and Immunity, Vol. 64, No. 10, 02.10.1996, p. 4105-4114.

Research output: Contribution to journalArticle

Kaneshiro, ES, Ellis, JE, Guo, Z, Jayasimhulu, K, Nicholas Maiorano, J & Kallam, KA 1996, 'Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii', Infection and Immunity, vol. 64, no. 10, pp. 4105-4114.
Kaneshiro ES, Ellis JE, Guo Z, Jayasimhulu K, Nicholas Maiorano J, Kallam KA. Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii. Infection and Immunity. 1996 Oct 2;64(10):4105-4114.
Kaneshiro, Edna S. ; Ellis, Jayne E. ; Guo, Zengkui ; Jayasimhulu, Koka ; Nicholas Maiorano, J. ; Kallam, Kathryn A. / Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii. In: Infection and Immunity. 1996 ; Vol. 64, No. 10. pp. 4105-4114.
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