TY - JOUR
T1 - Characterization of two novel human retropseudogenes related to the calmodulin-encoding gene, CaMII
AU - Koller, Markus
AU - Baumer, Alessandra
AU - Strehler, Emanuel E.
N1 - Funding Information:
This work was supported by a grant from the Swiss NSF (No. 3.112-0.88). E.E.S. is the recipient of a START research career development award from the Swiss NSF.
PY - 1991/1/15
Y1 - 1991/1/15
N2 - Two human genomic clones (λhg22 and λhg29), containing two novel calmodulin (CaM) retropseudogenes, were isolated and characterized. The two pseudogenes show high similarity with the human CaMII cDNA, hCE1 [SenGupta et al., J. Biol. Chem. 262 (1987) 16663-16670] and the CaMII-type retropseudogene, hCE2 (CaMII-ψ1) [SenGupta et al., Nucleic Acids Res. 17 (1989) 2868]. One of them, in clone λhg22 (CaMII-ψ2), shows all the characteristics of a processed pseudogene. In clone λhg29 (CaMII-ψ3), however, an Alu repetitive sequence was detected immediately upstream from the ancestral 5'-untranslated region. Downstream from the truncated 3'-untranslated region, three additional copies of Alu repetitive sequences flanking about 750 nucleotides of unknown origin were found. Such a processed retropseudogene flanked by multiple Alu repeats may be a target for further recombination events. The three retropseudogenes CaMII-ψ1, CaMII-ψ2 and CaMII-ψ3 are estimated to be about 49, 21 and 25 million years old, respectively.
AB - Two human genomic clones (λhg22 and λhg29), containing two novel calmodulin (CaM) retropseudogenes, were isolated and characterized. The two pseudogenes show high similarity with the human CaMII cDNA, hCE1 [SenGupta et al., J. Biol. Chem. 262 (1987) 16663-16670] and the CaMII-type retropseudogene, hCE2 (CaMII-ψ1) [SenGupta et al., Nucleic Acids Res. 17 (1989) 2868]. One of them, in clone λhg22 (CaMII-ψ2), shows all the characteristics of a processed pseudogene. In clone λhg29 (CaMII-ψ3), however, an Alu repetitive sequence was detected immediately upstream from the ancestral 5'-untranslated region. Downstream from the truncated 3'-untranslated region, three additional copies of Alu repetitive sequences flanking about 750 nucleotides of unknown origin were found. Such a processed retropseudogene flanked by multiple Alu repeats may be a target for further recombination events. The three retropseudogenes CaMII-ψ1, CaMII-ψ2 and CaMII-ψ3 are estimated to be about 49, 21 and 25 million years old, respectively.
KW - Alu repeat
KW - Recombinant DNA
KW - direct repeats
KW - gene family
KW - genomic clone
KW - repetitive sequence
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U2 - 10.1016/0378-1119(91)90058-J
DO - 10.1016/0378-1119(91)90058-J
M3 - Article
C2 - 1999288
AN - SCOPUS:0026063806
SN - 0378-1119
VL - 97
SP - 245
EP - 251
JO - Gene
JF - Gene
IS - 2
ER -