Characterization of the X-linked murine centrin Cetn2 gene

Peter E. Hart, Gregory M. Poynter, Clark M. Whitehead, James D. Orth, Janel N. Glantz, Robert C. Busby, Susan L. Barrett, Jeffrey L Salisbury

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A multi-gene family (Cetn1, Cetn2, and Cetn3) encodes the calcium-binding protein, centrin, in the mouse. This work characterizes the Cetn2 gene. Structurally, Cetn2 consists of five exons and four introns, and contains a classical TATA-less promoter. Cetn2 has two alternate transcription start sites, and a single length 3′ untranslated region. Fluorescence in situ hybridization demonstrates that Cetn2 is an X-linked gene whose alleles replicate asynchronously during S-phase. Cetn2 encodes a 172 amino acid protein, with a predicted molecular mass of 19,795 Da (pI = 4.71), that contains all of the defining characteristics of centrin. Northern blot analysis indicates that Cetn2 is ubiquitously expressed in the tissues of adult mice. RT-PCR shows that Cetn2 and Cetn3, but not Cetn1, are expressed in NIH 3T3 cells. Immunofluorescence microscopy demonstrates that mouse centrin 2 protein localizes to the region immediately surrounding the centrioles in the centrosome of NIH 3T3 cells.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalGene
Volume264
Issue number2
DOIs
StatePublished - Feb 21 2001

Fingerprint

NIH 3T3 Cells
Sulfamethoxazole Drug Combination Trimethoprim
Centrioles
X-Linked Genes
Centrosome
Calcium-Binding Proteins
Transcription Initiation Site
3' Untranslated Regions
Fluorescence In Situ Hybridization
S Phase
Fluorescence Microscopy
Northern Blotting
Introns
Genes
Exons
Alleles
Amino Acids
Polymerase Chain Reaction
Proteins
Mouse Cetn2 protein

Keywords

  • Centriole
  • Centrosome
  • Spermatogenesis
  • X-inactivation

ASJC Scopus subject areas

  • Genetics

Cite this

Hart, P. E., Poynter, G. M., Whitehead, C. M., Orth, J. D., Glantz, J. N., Busby, R. C., ... Salisbury, J. L. (2001). Characterization of the X-linked murine centrin Cetn2 gene. Gene, 264(2), 205-213. https://doi.org/10.1016/S0378-1119(01)00342-0

Characterization of the X-linked murine centrin Cetn2 gene. / Hart, Peter E.; Poynter, Gregory M.; Whitehead, Clark M.; Orth, James D.; Glantz, Janel N.; Busby, Robert C.; Barrett, Susan L.; Salisbury, Jeffrey L.

In: Gene, Vol. 264, No. 2, 21.02.2001, p. 205-213.

Research output: Contribution to journalArticle

Hart, PE, Poynter, GM, Whitehead, CM, Orth, JD, Glantz, JN, Busby, RC, Barrett, SL & Salisbury, JL 2001, 'Characterization of the X-linked murine centrin Cetn2 gene', Gene, vol. 264, no. 2, pp. 205-213. https://doi.org/10.1016/S0378-1119(01)00342-0
Hart PE, Poynter GM, Whitehead CM, Orth JD, Glantz JN, Busby RC et al. Characterization of the X-linked murine centrin Cetn2 gene. Gene. 2001 Feb 21;264(2):205-213. https://doi.org/10.1016/S0378-1119(01)00342-0
Hart, Peter E. ; Poynter, Gregory M. ; Whitehead, Clark M. ; Orth, James D. ; Glantz, Janel N. ; Busby, Robert C. ; Barrett, Susan L. ; Salisbury, Jeffrey L. / Characterization of the X-linked murine centrin Cetn2 gene. In: Gene. 2001 ; Vol. 264, No. 2. pp. 205-213.
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