Characterization of the X-linked murine centrin Cetn2 gene

Peter E. Hart, Gregory M. Poynter, Clark M. Whitehead, James D. Orth, Janel N. Glantz, Robert C. Busby, Susan L. Barrett, Jeffrey L. Salisbury

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A multi-gene family (Cetn1, Cetn2, and Cetn3) encodes the calcium-binding protein, centrin, in the mouse. This work characterizes the Cetn2 gene. Structurally, Cetn2 consists of five exons and four introns, and contains a classical TATA-less promoter. Cetn2 has two alternate transcription start sites, and a single length 3′ untranslated region. Fluorescence in situ hybridization demonstrates that Cetn2 is an X-linked gene whose alleles replicate asynchronously during S-phase. Cetn2 encodes a 172 amino acid protein, with a predicted molecular mass of 19,795 Da (pI = 4.71), that contains all of the defining characteristics of centrin. Northern blot analysis indicates that Cetn2 is ubiquitously expressed in the tissues of adult mice. RT-PCR shows that Cetn2 and Cetn3, but not Cetn1, are expressed in NIH 3T3 cells. Immunofluorescence microscopy demonstrates that mouse centrin 2 protein localizes to the region immediately surrounding the centrioles in the centrosome of NIH 3T3 cells.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalGene
Volume264
Issue number2
DOIs
StatePublished - Feb 21 2001

Keywords

  • Centriole
  • Centrosome
  • Spermatogenesis
  • X-inactivation

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Hart, P. E., Poynter, G. M., Whitehead, C. M., Orth, J. D., Glantz, J. N., Busby, R. C., Barrett, S. L., & Salisbury, J. L. (2001). Characterization of the X-linked murine centrin Cetn2 gene. Gene, 264(2), 205-213. https://doi.org/10.1016/S0378-1119(01)00342-0