Characterization of the tupaia rhabdovirus genome reveals a long open reading frame overlapping with P and a novel gene encoding a small hydrophobic protein

Christoph Springfeld, Gholamreza Darai, Roberto Cattaneo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Rhabdoviruses are negative-stranded RNA viruses of the order Mononegavirales and have been isolated from vertebrates, insects, and plants. Members of the genus Lyssavirus cause the invariably fatal disease rabies, and a member of the genus Vesiculovirus, Chandipura virus, has recently been associated with acute encephalitis in children. We present here the complete genome sequence and transcription map of a rhabdovirus isolated from cultivated cells of hepatocellular carcinoma tissue from a moribund tree shrew. The negative-strand genome of tupaia rhabdovirus is composed of 11,440 nucleotides and encodes six genes that are separated by one or two intergenic nucleotides. In addition to the typical rhabdovirus genes in the order N-P-M-G-L, a gene encoding a small hydrophobic putative type I transmembrane protein of approximately 11 kDa was identified between the M and G genes, and the corresponding transcript was detected in infected cells. Similar to some Vesiculoviruses and many Paramyxovirinae, the P gene has a second overlapping reading frame that can be accessed by ribosomal choice and encodes a protein of 26 kDa, predicted to be the largest C protein of these virus families. Phylogenetic analyses of the tupaia rhabdovirus N and L genes show that the virus is distantly related to the Vesiculoviruses, Ephemeroviruses, and the recently characterized Flanders virus and Oita virus and further extends the sequence territory occupied by animal rhabdoviruses.

Original languageEnglish (US)
Pages (from-to)6781-6790
Number of pages10
JournalJournal of Virology
Volume79
Issue number11
DOIs
StatePublished - Jun 2005

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Tupaia
Rhabdoviridae
Vesiculovirus
Open Reading Frames
open reading frames
Genome
genome
Genes
Viruses
Proteins
genes
proteins
Ephemerovirus
Flanders virus
viruses
Chandipura virus
Paramyxovirinae
Mononegavirales
Lyssavirus
Scandentia

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of the tupaia rhabdovirus genome reveals a long open reading frame overlapping with P and a novel gene encoding a small hydrophobic protein. / Springfeld, Christoph; Darai, Gholamreza; Cattaneo, Roberto.

In: Journal of Virology, Vol. 79, No. 11, 06.2005, p. 6781-6790.

Research output: Contribution to journalArticle

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