Characterization of the in vitro murine T-cell proliferative responses to murine and human thyroglobulins in thyroiditis-susceptible and -resistant mice

Laurel L. Simon, Christopher J. Krco, Chella S. David, Yi Chi M Kong

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Abstract

The in vitro proliferative response to autoantigenic mouse thyroglobulin (MTg) of lymph node cells (LNC) from thyroiditis-susceptible (high-responder) CBA/J (H-2k) mice was further characterized. The relatively weak response was enhanced by adding irradiated spleen cells from normal syngeneic mice to cultures of responding LNC. Furthermore, the adjuvant used for immunization was found to influence the magnitude of the response. Results of experiments varying both the adjuvant and the route of immunization (footpad versus subcutaneous) demonstrated that marked proliferative response to MTg in vitro was not necessarily a predictor of the severity of disease. However, the capacity to proliferate in response to MTg correlated with disease susceptibility, as reported previously. The response to MTg was dependent on Thy-1+, Lyt-1+2- cells and was inhibited by monoclonal I-A antibodies. Thus, proliferation is mediated by T cells of the helper/amplifier phenotype recognizing the autoantigen in association with Ia molecules. The determinants on human thyroglobulin (HTg) and MTg stimulating the proliferative responses of LNC from thyroiditis-susceptible and thyroiditis-resistant (low-responder) BALB/c (H-2d) mice were found to differ. Cells from resistant mice proliferated only in response to foreign determinants on HTg and not to shared or mouse-specific epitopes of MTg, whereas susceptible mice had T cells reactive to shared determinants expressed on MTg and HTg as well as to foreign determinants on HTg.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalCellular Immunology
Volume94
Issue number1
DOIs
StatePublished - 1985

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Thyroiditis
Thyroglobulin
T-Lymphocytes
Lymph Nodes
In Vitro Techniques
Immunization
Disease Susceptibility
Autoantigens
Helper-Inducer T-Lymphocytes
Epitopes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Characterization of the in vitro murine T-cell proliferative responses to murine and human thyroglobulins in thyroiditis-susceptible and -resistant mice. / Simon, Laurel L.; Krco, Christopher J.; David, Chella S.; Kong, Yi Chi M.

In: Cellular Immunology, Vol. 94, No. 1, 1985, p. 243-253.

Research output: Contribution to journalArticle

Simon, Laurel L. ; Krco, Christopher J. ; David, Chella S. ; Kong, Yi Chi M. / Characterization of the in vitro murine T-cell proliferative responses to murine and human thyroglobulins in thyroiditis-susceptible and -resistant mice. In: Cellular Immunology. 1985 ; Vol. 94, No. 1. pp. 243-253.
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