Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease

Dirk Chelius, Cheryl A. Conover, Michael A. Baldwin, E. Martin Spencer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by cultured adult human fibroblasts was recently identified as pregnancy-associated plasma protein-A (PAPP-A). In this study we showed that in addition to human IGFBP-4 the IGF-dependent IGFBP-4 protease also digests recombinant rat IGFBP-4 into two fragments by specifically cleaving at the carboxyl-terminal side of methionine at position 131 for rat IGFBP-4. Thus the cleavage site is at the KMKV site, which is not represented in other IGFBPs. While kallikrein may cleave at this site, its action is not specific.

Original languageEnglish (US)
Pages (from-to)360-366
Number of pages7
JournalGrowth Hormone and IGF Research
Volume10
Issue number6
DOIs
StatePublished - Jan 1 2000

Keywords

  • IGFBP-4
  • IGFBP-4 protease
  • Mass spectrometry
  • PAPP-A

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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