Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease

Dirk Chelius; Cheryl A. Conover; Michael A. Baldwin; E. Martin Spencer

doi:10.1054/ghir.2000.0177

Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease

Dirk Chelius, Cheryl A. Conover, Michael A. Baldwin, E. Martin Spencer

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by cultured adult human fibroblasts was recently identified as pregnancy-associated plasma protein-A (PAPP-A). In this study we showed that in addition to human IGFBP-4 the IGF-dependent IGFBP-4 protease also digests recombinant rat IGFBP-4 into two fragments by specifically cleaving at the carboxyl-terminal side of methionine at position 131 for rat IGFBP-4. Thus the cleavage site is at the KMKV site, which is not represented in other IGFBPs. While kallikrein may cleave at this site, its action is not specific.

Original language	English (US)
Pages (from-to)	360-366
Number of pages	7
Journal	Growth Hormone and IGF Research
Volume	10
Issue number	6
DOIs	https://doi.org/10.1054/ghir.2000.0177
State	Published - 2000

Keywords

IGFBP-4
IGFBP-4 protease
Mass spectrometry
PAPP-A

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1054/ghir.2000.0177

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title = "Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease",

abstract = "The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by cultured adult human fibroblasts was recently identified as pregnancy-associated plasma protein-A (PAPP-A). In this study we showed that in addition to human IGFBP-4 the IGF-dependent IGFBP-4 protease also digests recombinant rat IGFBP-4 into two fragments by specifically cleaving at the carboxyl-terminal side of methionine at position 131 for rat IGFBP-4. Thus the cleavage site is at the KMKV site, which is not represented in other IGFBPs. While kallikrein may cleave at this site, its action is not specific.",

keywords = "IGFBP-4, IGFBP-4 protease, Mass spectrometry, PAPP-A",

author = "Dirk Chelius and Conover, {Cheryl A.} and Baldwin, {Michael A.} and Spencer, {E. Martin}",

note = "Funding Information: 1Laboratory of Growth and Development, California Pacific Medical Center Research Institute, San Francisco, CA 94114, 2Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, MN 98195 and 3Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143-0446, USA",

year = "2000",

doi = "10.1054/ghir.2000.0177",

language = "English (US)",

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pages = "360--366",

journal = "Growth Hormone and IGF Research",

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T1 - Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease

AU - Chelius, Dirk

AU - Conover, Cheryl A.

AU - Baldwin, Michael A.

AU - Spencer, E. Martin

N1 - Funding Information: 1Laboratory of Growth and Development, California Pacific Medical Center Research Institute, San Francisco, CA 94114, 2Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, MN 98195 and 3Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143-0446, USA

PY - 2000

Y1 - 2000

N2 - The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by cultured adult human fibroblasts was recently identified as pregnancy-associated plasma protein-A (PAPP-A). In this study we showed that in addition to human IGFBP-4 the IGF-dependent IGFBP-4 protease also digests recombinant rat IGFBP-4 into two fragments by specifically cleaving at the carboxyl-terminal side of methionine at position 131 for rat IGFBP-4. Thus the cleavage site is at the KMKV site, which is not represented in other IGFBPs. While kallikrein may cleave at this site, its action is not specific.

AB - The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by cultured adult human fibroblasts was recently identified as pregnancy-associated plasma protein-A (PAPP-A). In this study we showed that in addition to human IGFBP-4 the IGF-dependent IGFBP-4 protease also digests recombinant rat IGFBP-4 into two fragments by specifically cleaving at the carboxyl-terminal side of methionine at position 131 for rat IGFBP-4. Thus the cleavage site is at the KMKV site, which is not represented in other IGFBPs. While kallikrein may cleave at this site, its action is not specific.

KW - IGFBP-4

KW - IGFBP-4 protease

KW - Mass spectrometry

KW - PAPP-A

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SN - 1096-6374

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Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease

Abstract

Keywords

ASJC Scopus subject areas

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