Characterization of the eIF2-associated protein p67 during brain ischemia and reperfusion

Cheri Owen, Christopher Lipinski, Andrea Page, Blaine White, Jonathon Sullivan, José Rafols, Gary Krause

Research output: Contribution to journalArticle

Abstract

Objectives: Within the first few minutes of reperfusion after global brain ischemia, there is a severe depression of protein translation owing to phosphorylation of the α-subunit of eukaryotic initiation factor 2 (eIF2). There is a 67 kDa peptide (p67) that, in its glycosylated form, binds to eIF2 and protects eIF2α from phosphorylation. Moreover, cells with high p67 content exhibit enhanced resistance to eIF2α phosphorylation. To examine the possibilities that deglycosylation of brain p67 occurs during ischemia and/or early reperfusion or that p67 deglycosylation may be more extensive in the vulnerable neurons, these experiments were undertaken to characterize the localization and activation state of p67 during early brain reperfusion Methods: Western blots using antibodies that recognize total p67, glycosylated p67 and phosphorylated eIF2α were used to characterize total p67 and glycosylated p67 during reperfusion-induced phosphorylation of eIF2α. We also characterized the immunohistochemical distribution of glycosylated p67 before and after brain ischemia and reperfusion. Results: There was a large increase in phosphorylated eIF2α, but there was no decrease in the levels of total or glycosylated p67 from those observed in controls following 10 minutes complete brain ischemia and 10 or 60 minutes subsequent reperfusion. Furthermore, there was no reduction in localized immunostaining for glycosylated p67 in vulnerable neurons during ischemia and reperfusion. Discussion: It does not appear that p67 plays a significant role in regulating the phosphorylation of eIF2α following transient brain ischemia.

Original languageEnglish (US)
Pages (from-to)818-821
Number of pages4
JournalNeurological research
Volume28
Issue number8
DOIs
StatePublished - Dec 1 2006

Keywords

  • Initiation factors
  • Transient brain ischemia
  • Translation
  • eIF2
  • p67

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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    Owen, C., Lipinski, C., Page, A., White, B., Sullivan, J., Rafols, J., & Krause, G. (2006). Characterization of the eIF2-associated protein p67 during brain ischemia and reperfusion. Neurological research, 28(8), 818-821. https://doi.org/10.1179/016164106X110418