Characterization of secophalloidin-induced force loss in cardiac myofibrils

Anna E. Bukatina, Gary C Sieck, Kenneth B. Campbell, Marek Belohlavek

Research output: Contribution to journalArticle

Abstract

Secophalloidin (SPH) is known to cause in cardiac myofibrils force without Ca2+ (half-maximal effect ∼2 mM) followed by irreversible loss of Ca2+-activated force. At maximal Ca2+ activation, SPH increases force (half-maximal effect < 0.1 mM). We found that SPH at low concentration (0.5 mM) did not cause either force activation or force loss at pCa 8.7, but both of these effects did occur when force was activated by Ca2+. The force loss was prevented when SPH was applied during rigor or in the presence of 2, 3-butanedione monoxime (85 mM). Furthermore, studying muscle in which the force was previously reduced by SPH (up to 50%) did not reveal significant changes in Ca2+ sensitivity and cooperativity of Ca2+-activation or qualitative alterations in SPH-induced changes in Ca2+-activated contraction. Data suggest that the force loss is mediated by cycling cross-bridges, and might reflect a reduction in force generated by individual cross-bridges.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalJournal of Muscle Research and Cell Motility
Volume30
Issue number5-6
DOIs
StatePublished - Sep 2009

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Myofibrils
Muscles
Chemical activation
Muscle
diacetylmonoxime

Keywords

  • Actomyosin
  • Ca regulation
  • Cardiac muscle
  • Muscle contraction
  • Myocardium
  • Myosin
  • Secophalloidin

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biochemistry

Cite this

Characterization of secophalloidin-induced force loss in cardiac myofibrils. / Bukatina, Anna E.; Sieck, Gary C; Campbell, Kenneth B.; Belohlavek, Marek.

In: Journal of Muscle Research and Cell Motility, Vol. 30, No. 5-6, 09.2009, p. 209-216.

Research output: Contribution to journalArticle

Bukatina, Anna E. ; Sieck, Gary C ; Campbell, Kenneth B. ; Belohlavek, Marek. / Characterization of secophalloidin-induced force loss in cardiac myofibrils. In: Journal of Muscle Research and Cell Motility. 2009 ; Vol. 30, No. 5-6. pp. 209-216.
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