Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures

Arno N. Vermeulen, Jan Van Deursen, Ruud H. Brakenhoff, Ton H W Lensen, Thivi Ponnudurai, Joseph H E Th Meuwissen

Research output: Contribution to journalArticle

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Abstract

Synchronised gametocyte cultures were used to study the biosynthesis of the sexual stage target antigens (Mr 230 000, 48 000 and 25 000) for anti gamete/zygote antibodies. These antigens were shown to be synthesized during gametocyte development from day 2-3 onwards until gametogenesis occurred. After gametogenesis a 25 kDa protein was predominantly synthesized, whereas synthesis of the other target proteins was hardly detectable. The 48, 45, and 25 kDa proteins appeared to be glycosylated, in addition the 25 kDa was also acylated in that it bound [3H]palmitic acid covalently. The iso-electric point (pI) of these proteins was assessed as being 6.0 ± 0.1 (for both 48 and 45 kDa) and 5.6 ± 0.1 (for 25 kDa).

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume20
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

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Keywords

  • Acylation
  • Gametocyte synchronization
  • Glycosylation
  • Plasmodium falciparum
  • Western blotting

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

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