Characterization of PCEng2, a β-1,3-endoglucanase homolog in Pneumocystis carinii with activity in cell wall regulation

Leah R. Villegas, Theodore J. Kottom, Andrew Harold Limper

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pneumocystis jirovecii pneumonia is an opportunistic fungal infection that causes severe respiratory impairment in immunocompromised patients. The viability of Pneumocystis organisms is dependent on the cyst cell wall, a structural feature that is regulated by essential cell wall-associated enzymes. The formation of the glucan-rich cystic wall has been previously characterized, but glucan degradation in the organism - specifically, degradation during trophic excystment - is not yet fully understood.Most studies of basic Pneumocystis biology have been conducted in Pneumocystis carinii or Pneumocystis murina, the varieties of this genus that infect rats and mice, respectively. Furthermore, all known treatments for P. jirovecii were initially discovered through studies of P. carinii. Accordingly, in this study, wehave identified a P.carinii β-1,3-endoglucanasegene (PCEng2) that is demonstrated to play a significant role in cell wall regulation. The cDNA sequence contained a 2.2-kb open reading frame with conserved amino acid domains homologous to similar fungal glycosyl hydrolases (GH family 81). The gene transcript showed up-regulation in cystic isolates, and the expressed protein was detected within both cyst and trophic forms. Complementation assays in Eng2-deleted Saccharomyces cerevisiae strains showed restoration of the cell wall separation defect during proliferation, demonstrating the importance of PCEng2 protein. during fungal growth. These findings suggest that regulation of cyst cell wall β-glucans is a fundamental process during completion of the Pneumocystis life cycle.

Original languageEnglish (US)
Pages (from-to)192-200
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume43
Issue number2
DOIs
StatePublished - Aug 1 2010

Fingerprint

Pneumocystis carinii
Pneumocystis
Cellulase
Cell Wall
Cells
Glucans
Cysts
Murinae
Degradation
Pneumocystis Pneumonia
Mycoses
Cell Separation
Opportunistic Infections
Immunocompromised Host
Hydrolases
Life Cycle Stages
Yeast
Open Reading Frames
Restoration
Saccharomyces cerevisiae

Keywords

  • β-1,3- endoglucanase
  • β-glucan
  • Cell wall
  • Glucanase
  • Pneumocystis carinii

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Characterization of PCEng2, a β-1,3-endoglucanase homolog in Pneumocystis carinii with activity in cell wall regulation. / Villegas, Leah R.; Kottom, Theodore J.; Limper, Andrew Harold.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 43, No. 2, 01.08.2010, p. 192-200.

Research output: Contribution to journalArticle

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