TY - JOUR
T1 - Characterization of HLA DR2 and DQ8 transgenic mouse with a new engineered mouse class II deletion, which lacks all endogenous class II genes
AU - Cheng, S.
AU - Smart, M.
AU - Hanson, J.
AU - David, C. S.
N1 - Funding Information:
The authors are greatly in debt to Drs Diane Mathis and Chris Benoist for providing us the Aβ 0/0 and MHCII Δ/Δ mice. The authors wish to thank Marshall Behrens, Drs Chris Krco and Govindarajan Rajagopalan for technical assistance. These studies are supported by the NIH grant AI 14764.
PY - 2003/11
Y1 - 2003/11
N2 - Human autoimmune diseases are a class of complex immune system disorders characterized by loss of tolerance to self-antigens. HLA class II molecules play a central role in the initiation, propagation and prolongation of the disease process. HLA class II transgenic mice with mouse endogenous class II gene Ab knockout were used successfully in several mouse models for human autoimmune diseases, such as IDDM, SLE and EAE in our Lab. However, these mice carry the functional mouse Eb gene from the Aβ0/0construct and could express Eβ/DRα(Eα) molecules and shape the T cell repertoire in these mice. Recently, we have obtained the new MHCII Δ/Δmice that are devoid of all endogenous conventional mouse MHC class II genes. When these mice are mated with our HLA class II transgenic mice, only human class II genes are expressed. The DR and DQ molecules expressed in these mice shape the T cell repertoire and regulate the immune response. Therefore, this new class of HLA transgenic mice is the first to be completely "humanized" in their MHC class II genes and will be an invaluable mouse model for human MHC class II associated autoimmune diseases.
AB - Human autoimmune diseases are a class of complex immune system disorders characterized by loss of tolerance to self-antigens. HLA class II molecules play a central role in the initiation, propagation and prolongation of the disease process. HLA class II transgenic mice with mouse endogenous class II gene Ab knockout were used successfully in several mouse models for human autoimmune diseases, such as IDDM, SLE and EAE in our Lab. However, these mice carry the functional mouse Eb gene from the Aβ0/0construct and could express Eβ/DRα(Eα) molecules and shape the T cell repertoire in these mice. Recently, we have obtained the new MHCII Δ/Δmice that are devoid of all endogenous conventional mouse MHC class II genes. When these mice are mated with our HLA class II transgenic mice, only human class II genes are expressed. The DR and DQ molecules expressed in these mice shape the T cell repertoire and regulate the immune response. Therefore, this new class of HLA transgenic mice is the first to be completely "humanized" in their MHC class II genes and will be an invaluable mouse model for human MHC class II associated autoimmune diseases.
KW - Autoimmune disease model
KW - Aβ
KW - HLA class II transgenic
KW - MHCII
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U2 - 10.1016/S0896-8411(03)00120-3
DO - 10.1016/S0896-8411(03)00120-3
M3 - Article
C2 - 14599844
AN - SCOPUS:0242655132
SN - 0896-8411
VL - 21
SP - 195
EP - 199
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
IS - 3
ER -