Characterization of HLA DR2 and DQ8 transgenic mouse with a new engineered mouse class II deletion, which lacks all endogenous class II genes

S. Cheng, M. Smart, J. Hanson, C. S. David

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Human autoimmune diseases are a class of complex immune system disorders characterized by loss of tolerance to self-antigens. HLA class II molecules play a central role in the initiation, propagation and prolongation of the disease process. HLA class II transgenic mice with mouse endogenous class II gene Ab knockout were used successfully in several mouse models for human autoimmune diseases, such as IDDM, SLE and EAE in our Lab. However, these mice carry the functional mouse Eb gene from the Aβ0/0construct and could express Eβ/DRα(Eα) molecules and shape the T cell repertoire in these mice. Recently, we have obtained the new MHCII Δ/Δmice that are devoid of all endogenous conventional mouse MHC class II genes. When these mice are mated with our HLA class II transgenic mice, only human class II genes are expressed. The DR and DQ molecules expressed in these mice shape the T cell repertoire and regulate the immune response. Therefore, this new class of HLA transgenic mice is the first to be completely "humanized" in their MHC class II genes and will be an invaluable mouse model for human MHC class II associated autoimmune diseases.

Original languageEnglish (US)
Pages (from-to)195-199
Number of pages5
JournalJournal of Autoimmunity
Volume21
Issue number3
DOIs
StatePublished - Nov 2003

Keywords

  • Autoimmune disease model
  • HLA class II transgenic
  • MHCII

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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