Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos

Anskar Y H Leung, Eric M. Mendenhall, Tommy T F Kwan, Raymond Liang, Craig Eckfeldt, Eleanor Chen, Matthias Hammerschmidt, Suzanne Grindley, Stephen C Ekker, Catherine M. Verfaillie

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be determined. In conclusion, the expanded ICM in Chd MO embryos represented an expansion of embryonic hematopoiesis that was skewed towards a monocytic lineage. BMP4, but not BMP2b or 7, was involved in this process. The results provide ground for further research into the mechanisms of embryonic hematopoietic cell expansion.

Original languageEnglish (US)
Pages (from-to)235-254
Number of pages20
JournalDevelopmental Biology
Volume277
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Zebrafish
Morpholinos
Embryonic Structures
Hematopoiesis
Fertilization
chordin
In Situ Hybridization
Blood Vessels
Transcription Factors
Phenotype
Gene Expression
Messenger RNA
Injections
Research

Keywords

  • BMP
  • Chordin
  • Differentiation
  • Gene expression
  • Hematopoiesis
  • Microarray
  • Morpholino
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Leung, A. Y. H., Mendenhall, E. M., Kwan, T. T. F., Liang, R., Eckfeldt, C., Chen, E., ... Verfaillie, C. M. (2005). Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos. Developmental Biology, 277(1), 235-254. https://doi.org/10.1016/j.ydbio.2004.09.032

Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos. / Leung, Anskar Y H; Mendenhall, Eric M.; Kwan, Tommy T F; Liang, Raymond; Eckfeldt, Craig; Chen, Eleanor; Hammerschmidt, Matthias; Grindley, Suzanne; Ekker, Stephen C; Verfaillie, Catherine M.

In: Developmental Biology, Vol. 277, No. 1, 01.01.2005, p. 235-254.

Research output: Contribution to journalArticle

Leung, AYH, Mendenhall, EM, Kwan, TTF, Liang, R, Eckfeldt, C, Chen, E, Hammerschmidt, M, Grindley, S, Ekker, SC & Verfaillie, CM 2005, 'Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos', Developmental Biology, vol. 277, no. 1, pp. 235-254. https://doi.org/10.1016/j.ydbio.2004.09.032
Leung AYH, Mendenhall EM, Kwan TTF, Liang R, Eckfeldt C, Chen E et al. Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos. Developmental Biology. 2005 Jan 1;277(1):235-254. https://doi.org/10.1016/j.ydbio.2004.09.032
Leung, Anskar Y H ; Mendenhall, Eric M. ; Kwan, Tommy T F ; Liang, Raymond ; Eckfeldt, Craig ; Chen, Eleanor ; Hammerschmidt, Matthias ; Grindley, Suzanne ; Ekker, Stephen C ; Verfaillie, Catherine M. / Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos. In: Developmental Biology. 2005 ; Vol. 277, No. 1. pp. 235-254.
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