Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and O. eichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH)

Huihuang Yan, Guoqing Liu, Zhukuan Cheng, Shaokai Min, Lihuang Zhu

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Restriction fragment length polymorphism (RFLP) analysis and GISH (genomic in situ hybridization) were performed on euploid plants derived from crosses between Oryza sativa (2n = 24, AA) and two brown planthopper-resistant accessions of O. eichingeri (2n = 24, CC). After screening with 164 RFLP markers, 60 of the 67 euploid plants were identified as introgression lines, each carrying 1-6 small O. eichingeri segments integrated on chromosomes 1, 2, 6, or 10. In the somatic chromosome preparations of F1 hybrid, O. eichingeri chromosomes, fluorescing greenish-yellow in the sequential GISH, appeared to be longer and to contain more heterochromatin than O. sativa ones, and this karyotypic polymorphism can be used to detect some introgressed O. eichingeri segments in euploid plants. In addition, GISH identification presented direct evidence for the transfer of small segments from O. eichingeri to O. sativa chromosome(s) which were subsequently recognized according to their condensation pattern, arm ratio, and chromosome length. The present results would contribute to the molecular mapping and selection of O. eichingeri - derived brown planthopper-resistant gene and positive yield QTLs.

Original languageEnglish (US)
Pages (from-to)86-95
Number of pages10
JournalGenome
Volume44
Issue number1
DOIs
StatePublished - Apr 11 2001

Keywords

  • Genomic in situ hybridization (GISH)
  • Introgression lines
  • Oryza eichingeri
  • Oryza sativa
  • RFLP

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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