Characterization of Epstein-Barr virus nuclear antigen(s) in different cell lines by radioimmunoelectrophoresis

T. B. Sculley, T. C. Spelsberg, G. R. Pearson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67,000 and 70,000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70,000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73,000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73,000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72,000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
JournalIntervirology
Volume22
Issue number4
StatePublished - 1984

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Epstein-Barr Virus Nuclear Antigens
Molecular Weight
Cell Line
Human Herpesvirus 4
Antigens
Cercopithecine Herpesvirus 1
Nuclear Proteins
B-Lymphocytes
Viruses
Infection

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of Epstein-Barr virus nuclear antigen(s) in different cell lines by radioimmunoelectrophoresis. / Sculley, T. B.; Spelsberg, T. C.; Pearson, G. R.

In: Intervirology, Vol. 22, No. 4, 1984, p. 191-200.

Research output: Contribution to journalArticle

Sculley, T. B. ; Spelsberg, T. C. ; Pearson, G. R. / Characterization of Epstein-Barr virus nuclear antigen(s) in different cell lines by radioimmunoelectrophoresis. In: Intervirology. 1984 ; Vol. 22, No. 4. pp. 191-200.
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