Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis

Thomas B. Sculley; Thomas C. Spelsherg; Gary R. Pearson

doi:10.1159/000149551

Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis

Thomas B. Sculley, Thomas C. Spelsherg, Gary R. Pearson

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67, 000 and 70, 000 were detected in NC₃₇ cells, while the Raji cell line contained an antigen with a molecular weight of 70, 000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73, 000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73, 000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72, 000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.

Original language	English (US)
Pages (from-to)	191-200
Number of pages	10
Journal	Intervirology
Volume	22
Issue number	4
DOIs	https://doi.org/10.1159/000149551
State	Published - 1984

Keywords

Epstein-Barr virus
Epstein-Barr virus nuclear antigen
Radioimmunoelectrophoresis

ASJC Scopus subject areas

Virology
Infectious Diseases

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10.1159/000149551

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title = "Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis",

abstract = "Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67, 000 and 70, 000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70, 000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73, 000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73, 000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72, 000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.",

keywords = "Epstein-Barr virus, Epstein-Barr virus nuclear antigen, Radioimmunoelectrophoresis",

author = "Sculley, {Thomas B.} and Spelsherg, {Thomas C.} and Pearson, {Gary R.}",

year = "1984",

doi = "10.1159/000149551",

language = "English (US)",

volume = "22",

pages = "191--200",

journal = "Intervirology",

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T1 - Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis

AU - Sculley, Thomas B.

AU - Spelsherg, Thomas C.

AU - Pearson, Gary R.

PY - 1984

Y1 - 1984

N2 - Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67, 000 and 70, 000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70, 000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73, 000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73, 000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72, 000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.

AB - Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67, 000 and 70, 000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70, 000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73, 000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73, 000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72, 000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.

KW - Epstein-Barr virus

KW - Epstein-Barr virus nuclear antigen

KW - Radioimmunoelectrophoresis

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Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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