Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis

Thomas B. Sculley, Thomas C. Spelsherg, Gary R. Pearson

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67, 000 and 70, 000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70, 000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73, 000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73, 000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72, 000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
JournalIntervirology
Volume22
Issue number4
DOIs
StatePublished - Jan 1 1984

Keywords

  • Epstein-Barr virus
  • Epstein-Barr virus nuclear antigen
  • Radioimmunoelectrophoresis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Characterization of epstein-barr virus nuclear antigen(S) in different cell lines by radioimmunoelectrophoresis'. Together they form a unique fingerprint.

Cite this