TY - JOUR
T1 - Characterization of cloned cDNA representing rat myelin basic protein
T2 - Absence of expression in brain of shiverer mutant mice
AU - Roach, Arthur
AU - Boylan, Kevin
AU - Horvath, Suzanna
AU - Prusiner, Stanley B.
AU - Hood, Leroy E.
N1 - Funding Information:
The authors are grateful to M. Cullen for the generous gift of three shiverer mice, to B. Wold for advice and assistance with the XgtlO vector, and to Karyl Minard for assistance in the preparation of sequencing gels, A. R. was supported by a postgraduate scholarship from the Natural Sciences and Engineering Research Council of Canada. This work was supported by National Institutes of Health grants to L. E. H. and S. B. P.
PY - 1983/10
Y1 - 1983/10
N2 - A cDNA library was constructed from mRNA isolated from the brains of 18-day-old rats, the age at which myelin biosynthesis is maximal. A synthetic DNA probe synthesized based on reverse translation of the amino acid sequence of rat myelin basic protein (MBP) was used to select two cDNA clones encoding MBP. A 1.5 kb Eco RI fragment from one clone was completely sequenced. When translated, a portion of this sequence was identical at 126 of 127 positions with the reported amino acid sequence for small MBP from the rat. Brains from mice of the homozygous shiverer genotype contained neatly reduced amounts of MBP mRNA relative to wild type. A deletion of MBP sequences in the genome of shiverer mice was also demonstrated. cDNAs for MBP will allow molecular investigation of the role this gene plays in both dysmyelinating and demyelinating diseases, as well as questions of MBP biosynthesis.
AB - A cDNA library was constructed from mRNA isolated from the brains of 18-day-old rats, the age at which myelin biosynthesis is maximal. A synthetic DNA probe synthesized based on reverse translation of the amino acid sequence of rat myelin basic protein (MBP) was used to select two cDNA clones encoding MBP. A 1.5 kb Eco RI fragment from one clone was completely sequenced. When translated, a portion of this sequence was identical at 126 of 127 positions with the reported amino acid sequence for small MBP from the rat. Brains from mice of the homozygous shiverer genotype contained neatly reduced amounts of MBP mRNA relative to wild type. A deletion of MBP sequences in the genome of shiverer mice was also demonstrated. cDNAs for MBP will allow molecular investigation of the role this gene plays in both dysmyelinating and demyelinating diseases, as well as questions of MBP biosynthesis.
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U2 - 10.1016/0092-8674(83)90536-6
DO - 10.1016/0092-8674(83)90536-6
M3 - Article
C2 - 6194889
AN - SCOPUS:0020509790
SN - 0092-8674
VL - 34
SP - 799
EP - 806
JO - Cell
JF - Cell
IS - 3
ER -