Characterization of cloned cDNA representing rat myelin basic protein

Absence of expression in brain of shiverer mutant mice

Arthur Roach, Kevin Boylan, Suzanna Horvath, Stanley B. Prusiner, Leroy E. Hood

Research output: Contribution to journalArticle

291 Citations (Scopus)

Abstract

A cDNA library was constructed from mRNA isolated from the brains of 18-day-old rats, the age at which myelin biosynthesis is maximal. A synthetic DNA probe synthesized based on reverse translation of the amino acid sequence of rat myelin basic protein (MBP) was used to select two cDNA clones encoding MBP. A 1.5 kb Eco RI fragment from one clone was completely sequenced. When translated, a portion of this sequence was identical at 126 of 127 positions with the reported amino acid sequence for small MBP from the rat. Brains from mice of the homozygous shiverer genotype contained neatly reduced amounts of MBP mRNA relative to wild type. A deletion of MBP sequences in the genome of shiverer mice was also demonstrated. cDNAs for MBP will allow molecular investigation of the role this gene plays in both dysmyelinating and demyelinating diseases, as well as questions of MBP biosynthesis.

Original languageEnglish (US)
Pages (from-to)799-806
Number of pages8
JournalCell
Volume34
Issue number3
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Myelin Basic Protein
Brain
Complementary DNA
Biosynthesis
Amino Acid Sequence
Clone Cells
Genes
Deoxyribonuclease EcoRI
Amino Acids
Messenger RNA
DNA Probes
Protein Biosynthesis
Demyelinating Diseases
Myelin Sheath
Gene Library
Rats
Genotype
rat Mbp protein
Genome

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Characterization of cloned cDNA representing rat myelin basic protein : Absence of expression in brain of shiverer mutant mice. / Roach, Arthur; Boylan, Kevin; Horvath, Suzanna; Prusiner, Stanley B.; Hood, Leroy E.

In: Cell, Vol. 34, No. 3, 1983, p. 799-806.

Research output: Contribution to journalArticle

Roach, Arthur ; Boylan, Kevin ; Horvath, Suzanna ; Prusiner, Stanley B. ; Hood, Leroy E. / Characterization of cloned cDNA representing rat myelin basic protein : Absence of expression in brain of shiverer mutant mice. In: Cell. 1983 ; Vol. 34, No. 3. pp. 799-806.
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