Characterization of cholecystokinin from the human brain

Laurence J Miller, I. Jardine, E. Weissman, V. L. Go, D. Speicher

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Human forms of cholecystokinin have not previously been characterized chemically. In this study, we have extracted and purified the predominant molecular form of cholecystokinin present in human cerebral cortex. The peptide was characterized by amino acid analysis, automated peptide sequencing, and fast atom bombardment mass spectrometry. It appears to be identical to porcine cholecystokinin-octapeptide, with the sequence of Asp-Tyr(SO3)-Met-Gly-Trp-Met-Asp-Phe(NH2). This structural identity is consistent with the observations that the peptide in human brain and porcine cholecystokinin-octapeptide are recognized similarly by a battery of antisera to porcine cholecystokinin; that they coelute from several chromatographic systems, including gel filtration, ion exchange, and reversed-phase; and that they possess similar biological activities.

Original languageEnglish (US)
Pages (from-to)835-840
Number of pages6
JournalJournal of Neurochemistry
Volume43
Issue number3
StatePublished - 1984

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Cholecystokinin
Sincalide
Brain
Swine
aspartyltyrosine
Peptides
Fast Atom Bombardment Mass Spectrometry
Ion Exchange
Bioactivity
Cerebral Cortex
Gel Chromatography
Mass spectrometry
Immune Sera
Ion exchange
Gels
Amino Acids
Atoms

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Miller, L. J., Jardine, I., Weissman, E., Go, V. L., & Speicher, D. (1984). Characterization of cholecystokinin from the human brain. Journal of Neurochemistry, 43(3), 835-840.

Characterization of cholecystokinin from the human brain. / Miller, Laurence J; Jardine, I.; Weissman, E.; Go, V. L.; Speicher, D.

In: Journal of Neurochemistry, Vol. 43, No. 3, 1984, p. 835-840.

Research output: Contribution to journalArticle

Miller, LJ, Jardine, I, Weissman, E, Go, VL & Speicher, D 1984, 'Characterization of cholecystokinin from the human brain', Journal of Neurochemistry, vol. 43, no. 3, pp. 835-840.
Miller LJ, Jardine I, Weissman E, Go VL, Speicher D. Characterization of cholecystokinin from the human brain. Journal of Neurochemistry. 1984;43(3):835-840.
Miller, Laurence J ; Jardine, I. ; Weissman, E. ; Go, V. L. ; Speicher, D. / Characterization of cholecystokinin from the human brain. In: Journal of Neurochemistry. 1984 ; Vol. 43, No. 3. pp. 835-840.
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