Characterization of B61, the ligand for the Eck receptor protein-tyrosine kinase

H. Shao, Akhilesh Pandey, K. S. O'Shea, M. Seldin, V. M. Dixit

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

B61 was originally described as a novel secreted tumor necrosis factor- α-inducible gene product in endothelial cells (Holzman, L. B., Marks, R. M., and Dixit, V. M. (1990) Mol. Cell. Biol. 10, 5836-5838). It was recently discovered that soluble recombinant B61 could serve as a ligand for the Eck receptor protein-tyrosine kinase, a member of the Eph/Eck subfamily of receptor protein tyrosine kinases (Bartley, T. D., Hunt, R. W., Welcher, A. A., Boyle, W. J., Parker, V. P., Lindberg, R. A., Lu, H. S., Colombero, A. M., Elliott, R. L., Guthrie, R. A., Hoist, P. L., Skrine, J. D., Toso, R. J., Zhang, M., Fernandez, E., Trail, G., Yarnum, B., Yarden, Y., Hunter, T., and Fox, G. M. (1994) Nature 368, 558-560). We now show that B61 can also exist as a cell surface glycosylphosphatidylinositol-linked protein that is capable of activating the Eck receptor protein-tyrosine kinase, the first such report of a receptor protein-tyrosine kinase ligand that is glycosylphosphatidylinositol-linked. In addition, the expression patterns of B61 and Eck during mouse ontogeny were determined by in situ hybridization. Both were found to be highly expressed in the developing lung and gut, while Eck was preferentially expressed in the thymus. Finally, the gene for B61 was localized to a specific position on mouse chromosome 3 by interspecific backcross analysis.

Original languageEnglish (US)
Pages (from-to)5636-5641
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number10
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Ephrin-A1
Receptor Protein-Tyrosine Kinases
GPI-Linked Proteins
Genes
Thymus
Glycosylphosphatidylinositols
Chromosomes, Human, Pair 3
Endothelial cells
Chromosomes
Thymus Gland
In Situ Hybridization
Membrane Proteins
Endothelial Cells
Tumor Necrosis Factor-alpha
Ligands
Lung

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of B61, the ligand for the Eck receptor protein-tyrosine kinase. / Shao, H.; Pandey, Akhilesh; O'Shea, K. S.; Seldin, M.; Dixit, V. M.

In: Journal of Biological Chemistry, Vol. 270, No. 10, 01.01.1995, p. 5636-5641.

Research output: Contribution to journalArticle

Shao, H. ; Pandey, Akhilesh ; O'Shea, K. S. ; Seldin, M. ; Dixit, V. M. / Characterization of B61, the ligand for the Eck receptor protein-tyrosine kinase. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 10. pp. 5636-5641.
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