Characterization of androgen receptor in Sertoli cell enriched testis

D. J. Tindall, D. A. Miller, A. R. Means

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The study of hormone action in the Sertoli cell in the above mentioned laboratory has been facilitated by development of a Sertoli cell enriched (SCE) testis which is devoid of germ cells. A cytoplasmic receptor for testosterone (T) was characterized in these Sertoli cell-enriched (SCE) testes from hypophysectomized (hypex) rats. Receptor binding was also measured in tubules isolated from SCE testes and primary cultures of Sertoli cells (33 fmol/10 6 cells). Receptor labeled at 0C with [ 3H]T± excess unlabeled T was precipitated with ammonium sulfate and specific binding was measured by gel filtration. Specific binding reached equilibrium within 2 hr and maximum binding was achieved at 3-6nM[ 3H]T. Scatchard analysis revealed a K[d] of 7.6 x 10 -10M which was similar to receptor preparations from non-irradiated hypex rats (6.8 x 10 -10M). However, the specific activity of receptors in non-irradiated hypex testis (12.7 fmol/mg prot) was greater than that in SCE hypex testis (9.6 fmol/mg prot). The receptor could be precipitated quantitatively at 40% ammonium sulfate, thereby separating ABP which precipitated at 50% and albumin which precipitated between 60 and 80%. The receptor was labile to heat, charcoal and sulfhydryl reagents. It had a 1/2 of several days, was excluded by Sephadex G-200, and migrated slower than ABP during polyacrylamide gel electrophoresis. Relative affinities for steroids were T>DHT, 5α- androstane-3α, 17β-diol>cyproterone acetate, 5α-androstane-3β, 17β-diol> androstenedione, dehydroepiandrosterone, cortisol.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalEndocrinology
Volume101
Issue number1
StatePublished - 1977
Externally publishedYes

Fingerprint

Sertoli Cells
Androgen Receptors
Testis
Androstane-3,17-diol
Ammonium Sulfate
Cyproterone Acetate
Sulfhydryl Reagents
Dehydroepiandrosterone
Androstenedione
Charcoal
Cytoplasmic and Nuclear Receptors
Germ Cells
Gel Chromatography
Hydrocortisone
Testosterone
Polyacrylamide Gel Electrophoresis
Albumins
Hot Temperature
Steroids
Hormones

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Tindall, D. J., Miller, D. A., & Means, A. R. (1977). Characterization of androgen receptor in Sertoli cell enriched testis. Endocrinology, 101(1), 13-23.

Characterization of androgen receptor in Sertoli cell enriched testis. / Tindall, D. J.; Miller, D. A.; Means, A. R.

In: Endocrinology, Vol. 101, No. 1, 1977, p. 13-23.

Research output: Contribution to journalArticle

Tindall, DJ, Miller, DA & Means, AR 1977, 'Characterization of androgen receptor in Sertoli cell enriched testis', Endocrinology, vol. 101, no. 1, pp. 13-23.
Tindall DJ, Miller DA, Means AR. Characterization of androgen receptor in Sertoli cell enriched testis. Endocrinology. 1977;101(1):13-23.
Tindall, D. J. ; Miller, D. A. ; Means, A. R. / Characterization of androgen receptor in Sertoli cell enriched testis. In: Endocrinology. 1977 ; Vol. 101, No. 1. pp. 13-23.
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