Characterization of an in vitro proliferation response to solubilized Trichinella spiralis antigens: Role of Ia antigens and Ly-1+ T cells

Christopher J. Krco; Chella S. David; Donald L. Wassom

doi:10.1016/0008-8749(82)90120-4

Characterization of an in vitro proliferation response to solubilized Trichinella spiralis antigens: Role of Ia antigens and Ly-1⁺ T cells

Christopher J. Krco, Chella S. David, Donald L. Wassom

Immunology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

An antigen extract from Trichinella spiralis muscle larvae was prepared and used to immunize strains of mice which were either relatively resistant (B10.S) or susceptible (B10.BR) to oral infection with T. spiralis larvae. Proliferation of cells from the draining lymph nodes was then measured in vitro after stimulation with the T. spiralis extract as well as appropriate control antigens. Primed cells from resistant B10.S mice responded better to challenge than did cells from the susceptible B10.BR strain. Cell-depletion experiments involving B10.S cells indicated that the in vitro cell proliferation response is dependent upon Ly-1⁺ T cells. The data were also consistent with a requirement for Ly-1⁺, -2⁺, and -3⁺ amplifier cells. Administration of anti-I^s serum to the cultures specifically inhibited (nearly 75%) the cell proliferation response. The potential applications of this system as a tool in immunogenetic analyses of immunity to T. spiralis are discussed.

Original language	English (US)
Pages (from-to)	359-367
Number of pages	9
Journal	Cellular Immunology
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(82)90120-4
State	Published - Apr 1982

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Immunology

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title = "Characterization of an in vitro proliferation response to solubilized Trichinella spiralis antigens: Role of Ia antigens and Ly-1+ T cells",

abstract = "An antigen extract from Trichinella spiralis muscle larvae was prepared and used to immunize strains of mice which were either relatively resistant (B10.S) or susceptible (B10.BR) to oral infection with T. spiralis larvae. Proliferation of cells from the draining lymph nodes was then measured in vitro after stimulation with the T. spiralis extract as well as appropriate control antigens. Primed cells from resistant B10.S mice responded better to challenge than did cells from the susceptible B10.BR strain. Cell-depletion experiments involving B10.S cells indicated that the in vitro cell proliferation response is dependent upon Ly-1+ T cells. The data were also consistent with a requirement for Ly-1+, -2+, and -3+ amplifier cells. Administration of anti-Is serum to the cultures specifically inhibited (nearly 75%) the cell proliferation response. The potential applications of this system as a tool in immunogenetic analyses of immunity to T. spiralis are discussed.",

author = "Krco, {Christopher J.} and David, {Chella S.} and Wassom, {Donald L.}",

note = "Funding Information: We thank Suresh Savarirayan for superb mouse husbandry and Mary Steege for preparation of the manuscript. This work was supported by NIH Grants CA-24473 and AI-17079.",

year = "1982",

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doi = "10.1016/0008-8749(82)90120-4",

language = "English (US)",

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journal = "Cellular Immunology",

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T2 - Role of Ia antigens and Ly-1+ T cells

AU - Krco, Christopher J.

AU - David, Chella S.

AU - Wassom, Donald L.

N1 - Funding Information: We thank Suresh Savarirayan for superb mouse husbandry and Mary Steege for preparation of the manuscript. This work was supported by NIH Grants CA-24473 and AI-17079.

PY - 1982/4

Y1 - 1982/4

N2 - An antigen extract from Trichinella spiralis muscle larvae was prepared and used to immunize strains of mice which were either relatively resistant (B10.S) or susceptible (B10.BR) to oral infection with T. spiralis larvae. Proliferation of cells from the draining lymph nodes was then measured in vitro after stimulation with the T. spiralis extract as well as appropriate control antigens. Primed cells from resistant B10.S mice responded better to challenge than did cells from the susceptible B10.BR strain. Cell-depletion experiments involving B10.S cells indicated that the in vitro cell proliferation response is dependent upon Ly-1+ T cells. The data were also consistent with a requirement for Ly-1+, -2+, and -3+ amplifier cells. Administration of anti-Is serum to the cultures specifically inhibited (nearly 75%) the cell proliferation response. The potential applications of this system as a tool in immunogenetic analyses of immunity to T. spiralis are discussed.

AB - An antigen extract from Trichinella spiralis muscle larvae was prepared and used to immunize strains of mice which were either relatively resistant (B10.S) or susceptible (B10.BR) to oral infection with T. spiralis larvae. Proliferation of cells from the draining lymph nodes was then measured in vitro after stimulation with the T. spiralis extract as well as appropriate control antigens. Primed cells from resistant B10.S mice responded better to challenge than did cells from the susceptible B10.BR strain. Cell-depletion experiments involving B10.S cells indicated that the in vitro cell proliferation response is dependent upon Ly-1+ T cells. The data were also consistent with a requirement for Ly-1+, -2+, and -3+ amplifier cells. Administration of anti-Is serum to the cultures specifically inhibited (nearly 75%) the cell proliferation response. The potential applications of this system as a tool in immunogenetic analyses of immunity to T. spiralis are discussed.

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Characterization of an in vitro proliferation response to solubilized Trichinella spiralis antigens: Role of Ia antigens and Ly-1⁺ T cells

Abstract

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