Characterization of an in vitro proliferation response to solubilized Trichinella spiralis antigens: Role of Ia antigens and Ly-1+ T cells

Christopher J. Krco, Chella S. David, Donald L. Wassom

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

An antigen extract from Trichinella spiralis muscle larvae was prepared and used to immunize strains of mice which were either relatively resistant (B10.S) or susceptible (B10.BR) to oral infection with T. spiralis larvae. Proliferation of cells from the draining lymph nodes was then measured in vitro after stimulation with the T. spiralis extract as well as appropriate control antigens. Primed cells from resistant B10.S mice responded better to challenge than did cells from the susceptible B10.BR strain. Cell-depletion experiments involving B10.S cells indicated that the in vitro cell proliferation response is dependent upon Ly-1+ T cells. The data were also consistent with a requirement for Ly-1+, -2+, and -3+ amplifier cells. Administration of anti-Is serum to the cultures specifically inhibited (nearly 75%) the cell proliferation response. The potential applications of this system as a tool in immunogenetic analyses of immunity to T. spiralis are discussed.

Original languageEnglish (US)
Pages (from-to)359-367
Number of pages9
JournalCellular Immunology
Volume68
Issue number2
DOIs
StatePublished - Apr 1982

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Characterization of an in vitro proliferation response to solubilized Trichinella spiralis antigens: Role of Ia antigens and Ly-1<sup>+</sup> T cells'. Together they form a unique fingerprint.

  • Cite this