Characterization of an androgen binding protein (ABP) in rat testis and epididymis

E. Martin Ritzén, M. Catherine Dobbins, Donald J. Tindall, Frank S. French, Shihadeh N. Nayfeh

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

An androgen binding protein (ABP) was demonstrated in the 105,000 g supernatant of rat testis homogenate after charcoal extraction of endogenous steroids. Testis ABP proved to be identical to an ABP previously described in rat epididymis. It contained saturable high-affinity sites which exhibited binding specificity for dihydrotestosterone (6) and testosterone when measured by polyacrylamide gel electrophoresis or by competitive binding using charcoal adsorption. Binding to ABP was not affected by ribonuclease or neuraminidase but was decreased by the disulfide reducing agent, dithiothreitol and the sulfhydryl reagent, N-ethylmaleimide. Binding was abolished by treatment with proteolytic enzyme. The mean molecular radius of ABP was 2.92 nm as determined by the retardation of electrophoretic mobility in polyacrylamide gels of decreasing pore size. Assuming a partial specific volume of 0.66-0.74 the molecular weight was 86,000-91,000 for a spherical molecule. ABP binding was stable after treating at 45° C for 20 min. but was destroyed at 60° C. Binding was maximal between pH 7.5 and 9.0 and decreased at pH below 7.0.

Original languageEnglish (US)
Pages (from-to)593-607
Number of pages15
JournalSteroids
Volume21
Issue number4
DOIs
StatePublished - Apr 1973

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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    Ritzén, E. M., Dobbins, M. C., Tindall, D. J., French, F. S., & Nayfeh, S. N. (1973). Characterization of an androgen binding protein (ABP) in rat testis and epididymis. Steroids, 21(4), 593-607. https://doi.org/10.1016/0039-128X(73)90047-0