Characterization of a novel Src-like adapter protein that associates with the Eck receptor tyrosine kinase

Akhilesh Pandey, H. Duan, V. M. Dixit

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The Eph family of receptor protein tyrosine kinases (RPTKs) is the largest family of RPTKs. The signal transduction pathways initiated by this family have only recently begun to be explored. Using a yeast two-hybrid screen to identify molecules that interact with the cytoplasmic domain of Eck, it was previously shown that activated Eck RPTK bound to and stimulated phosphatidylinositol 3-kinase (Pandey, A., Lazar, D. F., Saltiel, A. R., and Dixit, V. M. (1994) J. Biol. Chem. 269, 3015430157). Also isolated from this same screen was a novel protein containing SH3 and SH2 adapter modules that had striking homology to those found in the Src family of non-receptor tyrosine kinases. However, unlike other Src family members, it lacked a catalytic tyrosine kinase domain. Hence, this protein was designated SLAP for Src-like adapter protein. Using glutathione S-transferase fusion proteins, it was demonstrated that SLAP bound to activated Eck receptor tyrosine kinase. Therefore, SLAP is a novel candidate downstream signaling intermediate and the first member of the Src family that resembles an adapter molecule.

Original languageEnglish (US)
Pages (from-to)19201-19204
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number33
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Receptor Protein-Tyrosine Kinases
Protein-Tyrosine Kinases
Proteins
Eph Family Receptors
Phosphatidylinositol 3-Kinase
Signal transduction
Molecules
Glutathione Transferase
Yeast
Signal Transduction
Fusion reactions
Yeasts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of a novel Src-like adapter protein that associates with the Eck receptor tyrosine kinase. / Pandey, Akhilesh; Duan, H.; Dixit, V. M.

In: Journal of Biological Chemistry, Vol. 270, No. 33, 01.01.1995, p. 19201-19204.

Research output: Contribution to journalArticle

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