Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves

Bobby L. Boyanton; Amro Almradi; Mark J. Espy; Anne E. Prada; John P. Gibson; Bobbi S. Pritt

doi:10.1128/JCM.03062-13

Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves

Bobby L. Boyanton, Amro Almradi, Mark J. Espy, Anne E. Prada, John P. Gibson, Bobbi S. Pritt

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.

Original language	English (US)
Pages (from-to)	957-959
Number of pages	3
Journal	Journal of clinical microbiology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1128/JCM.03062-13
State	Published - Mar 2014

ASJC Scopus subject areas

Microbiology (medical)

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Boyanton, B. L., Almradi, A., Espy, M. J., Prada, A. E., Gibson, J. P., & Pritt, B. S. (2014). Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves. Journal of clinical microbiology, 52(3), 957-959. https://doi.org/10.1128/JCM.03062-13

Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves. / Boyanton, Bobby L.; Almradi, Amro; Espy, Mark J. et al.
In: Journal of clinical microbiology, Vol. 52, No. 3, 03.2014, p. 957-959.

Research output: Contribution to journal › Article › peer-review

Boyanton, BL, Almradi, A, Espy, MJ, Prada, AE, Gibson, JP & Pritt, BS 2014, 'Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves', Journal of clinical microbiology, vol. 52, no. 3, pp. 957-959. https://doi.org/10.1128/JCM.03062-13

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abstract = "We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.",

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Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves

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