Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves

Bobby L. Boyanton, Amro Almradi, Mark J. Espy, Anne E. Prada, John P. Gibson, Bobbi S. Pritt

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.

Original languageEnglish (US)
Pages (from-to)957-959
Number of pages3
JournalJournal of Clinical Microbiology
Volume52
Issue number3
DOIs
StatePublished - 2014

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Human Herpesvirus 2
Human Herpesvirus 1
Real-Time Polymerase Chain Reaction
Binding Sites

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay : Frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves. / Boyanton, Bobby L.; Almradi, Amro; Espy, Mark J.; Prada, Anne E.; Gibson, John P.; Pritt, Bobbi S.

In: Journal of Clinical Microbiology, Vol. 52, No. 3, 2014, p. 957-959.

Research output: Contribution to journalArticle

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