Characterization of a new human multiple myeloma cell line, UMJF-2, which suppresses antibody production by B-lymphocytes in vitro

John P. Farnen, Michael Tyrkus, Curtis A. Hanson, Robert L. Cody, Stephen G. Emerson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A new human plasma cell line, UMJF-2, has been derived from the bone marrow of a patient with multiple myeloma. Morphological studies disclosed large nucleoli, moderate numbers of mitochondria, and scant endoplasmic reticulum consistent with a plasmablastic morphology. The cells have immunologic characteristics of early plasma cells, including intense expression of cytoplasmic IgG-λ and weaker, but discernible, expression of surface IgG-λ. Cell surface antigens defined by the monoclonal antibodies OKT10 (CD38) and PCA-1, characteristic of mature plasma cells, and B1 (CD20), B4 (CD19), and I-2 (HLA-DR), characteristic of earlier stages of B-lymphocyte differentiation, are present on UMJF-2 cells. Cytogenetic studies reveal the presence of trisomy 12. UMJF-2 does not contain the Epstein-Barr virus by Southern blot analysis. Tissue culture media conditioned by these cells contains a soluble immunosuppressive factor, capable of inhibiting pokeweed mitogen induced IgM secretion by normal human B-lymphocytes. UMJF-2 provides a model for the study of the pathogenesis of polyclonal hypogammaglobulinemia in human multiple myeloma.

Original languageEnglish (US)
Pages (from-to)574-584
Number of pages11
JournalLeukemia
Volume5
Issue number7
StatePublished - Jul 1991
Externally publishedYes

Fingerprint

Plasma Cells
Multiple Myeloma
Antibody Formation
B-Lymphocytes
Cell Line
Immunoglobulin G
Agammaglobulinemia
Pokeweed Mitogens
Passive Cutaneous Anaphylaxis
Trisomy
HLA-DR Antigens
Surface Antigens
Immunosuppressive Agents
Conditioned Culture Medium
Southern Blotting
Human Herpesvirus 4
Cytogenetics
Endoplasmic Reticulum
Immunoglobulin M
Mitochondria

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Farnen, J. P., Tyrkus, M., Hanson, C. A., Cody, R. L., & Emerson, S. G. (1991). Characterization of a new human multiple myeloma cell line, UMJF-2, which suppresses antibody production by B-lymphocytes in vitro. Leukemia, 5(7), 574-584.

Characterization of a new human multiple myeloma cell line, UMJF-2, which suppresses antibody production by B-lymphocytes in vitro. / Farnen, John P.; Tyrkus, Michael; Hanson, Curtis A.; Cody, Robert L.; Emerson, Stephen G.

In: Leukemia, Vol. 5, No. 7, 07.1991, p. 574-584.

Research output: Contribution to journalArticle

Farnen, JP, Tyrkus, M, Hanson, CA, Cody, RL & Emerson, SG 1991, 'Characterization of a new human multiple myeloma cell line, UMJF-2, which suppresses antibody production by B-lymphocytes in vitro', Leukemia, vol. 5, no. 7, pp. 574-584.
Farnen, John P. ; Tyrkus, Michael ; Hanson, Curtis A. ; Cody, Robert L. ; Emerson, Stephen G. / Characterization of a new human multiple myeloma cell line, UMJF-2, which suppresses antibody production by B-lymphocytes in vitro. In: Leukemia. 1991 ; Vol. 5, No. 7. pp. 574-584.
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