Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

Beth F. Ladin; Elizabeth E. Murray; Amy C. Halling; Kevin C. Halling; Nanda Tilakaratne; George L. Long; L. L. Houston; Robert F. Weaver

doi:10.1007/BF00166464

Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

Beth F. Ladin, Elizabeth E. Murray, Amy C. Halling, Kevin C. Halling, Nanda Tilakaratne, George L. Long, L. L. Houston, Robert F. Weaver

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain.

Original language	English (US)
Pages (from-to)	287-295
Number of pages	9
Journal	Plant Molecular Biology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1007/BF00166464
State	Published - May 1987

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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title = "Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis",

abstract = "Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain.",

author = "Ladin, {Beth F.} and Murray, {Elizabeth E.} and Halling, {Amy C.} and Halling, {Kevin C.} and Nanda Tilakaratne and Long, {George L.} and Houston, {L. L.} and Weaver, {Robert F.}",

year = "1987",

month = may,

doi = "10.1007/BF00166464",

language = "English (US)",

volume = "9",

pages = "287--295",

journal = "Plant Molecular Biology",

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T1 - Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

AU - Ladin, Beth F.

AU - Murray, Elizabeth E.

AU - Halling, Amy C.

AU - Halling, Kevin C.

AU - Tilakaratne, Nanda

AU - Long, George L.

AU - Houston, L. L.

AU - Weaver, Robert F.

PY - 1987/5

Y1 - 1987/5

N2 - Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain.

AB - Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain.

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Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

Abstract

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