Characterization of 10 vulvar carcinoma cell lines by karyotyping, comparative genomic hybridization and flow cytometry

Misa Raitanen, Maria J. Worsham, Taina Lakkala, Thomas E. Carey, Daniel L. Van Dyke, Reidar Grénman, Pekka Klemi, Virpi Rantanen, Jorma Isola, Seija Grénman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective and methods. Ten vulvar squamous cell carcinoma cell lines established at the University of Michigan (UM-SCV-1A, -1B, -2, -3, -4, -6, -7) and at the University of Turku (UT-SCV-1, -2, -3) were characterized by G-banding karyotyping, comparative genomic hybridization (CGH), and deoxyribonucleic acid (DNA) flow cytometry. Results. All cell lines had hyperdiploid DNA content as measured by flow cytometry. The DNA index (DI) remained relatively stable through different passages in 9 of 10 cases. DIs of UM-SCV-3 and UT-SCV-2 were near-diploid, as were the corresponding karyotypes. The 10 SCVs showed remarkable genetic similarities with respect to consistent chromosome rearrangements. Loss of 3p, noted in 8/10 SCVs, was narrowed to the smallest common region at 3p11-3p13. Loss of 8pter-p11 was observed in 10/10 cell lines. Loss of 11qter-q23 was present in UM-SCV-1 and -2, and in all four recently karyotyped SCVs. Other consistent losses include Xpter-p11 in 6/10, and 18qter-q11 in 7/10 cell lines. Common gains included gain of 8q in 8/10 and 3q in 6/10. Consistent copy number imbalances were confirmed by CGH; concerning loss of 3p, in 63%, to loss of 8p in 70%, to gain of 3q in 83%, and to gain of 8q in 75% of the cell lines. Conclusions. CGH and karyotyping showed concordance in defining copy number imbalances, thus supporting the accuracy of CGH to detect chromosome imbalances in tumors that cannot be karyotyped.

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalGynecologic Oncology
Volume93
Issue number1
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Karyotyping
Comparative Genomic Hybridization
Flow Cytometry
Carcinoma
Cell Line
DNA
Chromosomes
Polyploidy
Diploidy
Karyotype
Squamous Cell Carcinoma
Neoplasms

Keywords

  • CGH
  • Characterization
  • Chromosomal aberrations
  • Cytogenetics
  • Squamous cell carcinoma
  • Vulvar carcinoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Raitanen, M., Worsham, M. J., Lakkala, T., Carey, T. E., Van Dyke, D. L., Grénman, R., ... Grénman, S. (2004). Characterization of 10 vulvar carcinoma cell lines by karyotyping, comparative genomic hybridization and flow cytometry. Gynecologic Oncology, 93(1), 155-163. https://doi.org/10.1016/j.ygyno.2003.12.033

Characterization of 10 vulvar carcinoma cell lines by karyotyping, comparative genomic hybridization and flow cytometry. / Raitanen, Misa; Worsham, Maria J.; Lakkala, Taina; Carey, Thomas E.; Van Dyke, Daniel L.; Grénman, Reidar; Klemi, Pekka; Rantanen, Virpi; Isola, Jorma; Grénman, Seija.

In: Gynecologic Oncology, Vol. 93, No. 1, 04.2004, p. 155-163.

Research output: Contribution to journalArticle

Raitanen, M, Worsham, MJ, Lakkala, T, Carey, TE, Van Dyke, DL, Grénman, R, Klemi, P, Rantanen, V, Isola, J & Grénman, S 2004, 'Characterization of 10 vulvar carcinoma cell lines by karyotyping, comparative genomic hybridization and flow cytometry', Gynecologic Oncology, vol. 93, no. 1, pp. 155-163. https://doi.org/10.1016/j.ygyno.2003.12.033
Raitanen, Misa ; Worsham, Maria J. ; Lakkala, Taina ; Carey, Thomas E. ; Van Dyke, Daniel L. ; Grénman, Reidar ; Klemi, Pekka ; Rantanen, Virpi ; Isola, Jorma ; Grénman, Seija. / Characterization of 10 vulvar carcinoma cell lines by karyotyping, comparative genomic hybridization and flow cytometry. In: Gynecologic Oncology. 2004 ; Vol. 93, No. 1. pp. 155-163.
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abstract = "Objective and methods. Ten vulvar squamous cell carcinoma cell lines established at the University of Michigan (UM-SCV-1A, -1B, -2, -3, -4, -6, -7) and at the University of Turku (UT-SCV-1, -2, -3) were characterized by G-banding karyotyping, comparative genomic hybridization (CGH), and deoxyribonucleic acid (DNA) flow cytometry. Results. All cell lines had hyperdiploid DNA content as measured by flow cytometry. The DNA index (DI) remained relatively stable through different passages in 9 of 10 cases. DIs of UM-SCV-3 and UT-SCV-2 were near-diploid, as were the corresponding karyotypes. The 10 SCVs showed remarkable genetic similarities with respect to consistent chromosome rearrangements. Loss of 3p, noted in 8/10 SCVs, was narrowed to the smallest common region at 3p11-3p13. Loss of 8pter-p11 was observed in 10/10 cell lines. Loss of 11qter-q23 was present in UM-SCV-1 and -2, and in all four recently karyotyped SCVs. Other consistent losses include Xpter-p11 in 6/10, and 18qter-q11 in 7/10 cell lines. Common gains included gain of 8q in 8/10 and 3q in 6/10. Consistent copy number imbalances were confirmed by CGH; concerning loss of 3p, in 63{\%}, to loss of 8p in 70{\%}, to gain of 3q in 83{\%}, and to gain of 8q in 75{\%} of the cell lines. Conclusions. CGH and karyotyping showed concordance in defining copy number imbalances, thus supporting the accuracy of CGH to detect chromosome imbalances in tumors that cannot be karyotyped.",
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T1 - Characterization of 10 vulvar carcinoma cell lines by karyotyping, comparative genomic hybridization and flow cytometry

AU - Raitanen, Misa

AU - Worsham, Maria J.

AU - Lakkala, Taina

AU - Carey, Thomas E.

AU - Van Dyke, Daniel L.

AU - Grénman, Reidar

AU - Klemi, Pekka

AU - Rantanen, Virpi

AU - Isola, Jorma

AU - Grénman, Seija

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N2 - Objective and methods. Ten vulvar squamous cell carcinoma cell lines established at the University of Michigan (UM-SCV-1A, -1B, -2, -3, -4, -6, -7) and at the University of Turku (UT-SCV-1, -2, -3) were characterized by G-banding karyotyping, comparative genomic hybridization (CGH), and deoxyribonucleic acid (DNA) flow cytometry. Results. All cell lines had hyperdiploid DNA content as measured by flow cytometry. The DNA index (DI) remained relatively stable through different passages in 9 of 10 cases. DIs of UM-SCV-3 and UT-SCV-2 were near-diploid, as were the corresponding karyotypes. The 10 SCVs showed remarkable genetic similarities with respect to consistent chromosome rearrangements. Loss of 3p, noted in 8/10 SCVs, was narrowed to the smallest common region at 3p11-3p13. Loss of 8pter-p11 was observed in 10/10 cell lines. Loss of 11qter-q23 was present in UM-SCV-1 and -2, and in all four recently karyotyped SCVs. Other consistent losses include Xpter-p11 in 6/10, and 18qter-q11 in 7/10 cell lines. Common gains included gain of 8q in 8/10 and 3q in 6/10. Consistent copy number imbalances were confirmed by CGH; concerning loss of 3p, in 63%, to loss of 8p in 70%, to gain of 3q in 83%, and to gain of 8q in 75% of the cell lines. Conclusions. CGH and karyotyping showed concordance in defining copy number imbalances, thus supporting the accuracy of CGH to detect chromosome imbalances in tumors that cannot be karyotyped.

AB - Objective and methods. Ten vulvar squamous cell carcinoma cell lines established at the University of Michigan (UM-SCV-1A, -1B, -2, -3, -4, -6, -7) and at the University of Turku (UT-SCV-1, -2, -3) were characterized by G-banding karyotyping, comparative genomic hybridization (CGH), and deoxyribonucleic acid (DNA) flow cytometry. Results. All cell lines had hyperdiploid DNA content as measured by flow cytometry. The DNA index (DI) remained relatively stable through different passages in 9 of 10 cases. DIs of UM-SCV-3 and UT-SCV-2 were near-diploid, as were the corresponding karyotypes. The 10 SCVs showed remarkable genetic similarities with respect to consistent chromosome rearrangements. Loss of 3p, noted in 8/10 SCVs, was narrowed to the smallest common region at 3p11-3p13. Loss of 8pter-p11 was observed in 10/10 cell lines. Loss of 11qter-q23 was present in UM-SCV-1 and -2, and in all four recently karyotyped SCVs. Other consistent losses include Xpter-p11 in 6/10, and 18qter-q11 in 7/10 cell lines. Common gains included gain of 8q in 8/10 and 3q in 6/10. Consistent copy number imbalances were confirmed by CGH; concerning loss of 3p, in 63%, to loss of 8p in 70%, to gain of 3q in 83%, and to gain of 8q in 75% of the cell lines. Conclusions. CGH and karyotyping showed concordance in defining copy number imbalances, thus supporting the accuracy of CGH to detect chromosome imbalances in tumors that cannot be karyotyped.

KW - CGH

KW - Characterization

KW - Chromosomal aberrations

KW - Cytogenetics

KW - Squamous cell carcinoma

KW - Vulvar carcinoma

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