Characterization and gene expression profiling in glioma cell lines with deletion of chromosome 19 before and after microcell-mediated restoration of normal human chromosome 19

Kristen L. Drucker, Gaspar J. Kitange, Thomas M. Kollmeyer, Mark E. Law, Sandra Passe, Amanda L. Rynearson, Hilary Blair, Cheryl L. Soderberg, Bruce W. Morlan, Karla V. Ballman, Caterina Giannini, Robert Brian Jenkins

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nearly 10% of human gliomas are oligodendrogliomas. Deletion of chromosome arm 19q, often in conjunction with deletion of 1p, has been observed in 65-80% of these tumors. This has suggested the presence of a tumor suppressor gene located on the 19q arm. Chromosome 19 deletion is also of interest due to the better prognosis of patients with deletion, including longer survival and better response to chemotherapy, compared with patients without deletion. Two glioma cell lines with deletion of 19q were used for chromosome 19 microcell-mediated transfer, to assess the effect of replacing the deleted segment. Complementation with chromosome 19 significantly reduced the growth rate of the hybrid cells compared with the parental cell lines. Affymetrix U133 Plus 2.0 Gene Chip analysis was performed to measure and compare the expression of the chromosome 19 genes in the chromosome 19 hybrid cell lines to the parental cell line. Probes were considered significantly different when a P value <0.01 was seen in all of the cell line comparisons. Of 345 probes within the commonly deleted 19q region, seven genes (APOE, RCN3, FLJ10781, SAE1, STRN4, CCDC8, and BCL2L12) were identified as potential candidate genes. RT-PCR analysis of primary tumor specimens showed that several genes had significant differences when stratified by tumor morphology or deletion status. This suggests that one or more of these candidates may play a role in glioma formation or progression.

Original languageEnglish (US)
Pages (from-to)854-864
Number of pages11
JournalGenes Chromosomes and Cancer
Volume48
Issue number10
DOIs
StatePublished - Oct 2009

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Chromosomes, Human, Pair 19
Human Chromosomes
Gene Expression Profiling
Glioma
Cell Line
Chromosome Deletion
Hybrid Cells
Genes
Oligodendroglioma
Neoplasms
Apolipoproteins E
Oligonucleotide Array Sequence Analysis
Tumor Suppressor Genes
Drug Therapy
Polymerase Chain Reaction
Survival
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Characterization and gene expression profiling in glioma cell lines with deletion of chromosome 19 before and after microcell-mediated restoration of normal human chromosome 19. / Drucker, Kristen L.; Kitange, Gaspar J.; Kollmeyer, Thomas M.; Law, Mark E.; Passe, Sandra; Rynearson, Amanda L.; Blair, Hilary; Soderberg, Cheryl L.; Morlan, Bruce W.; Ballman, Karla V.; Giannini, Caterina; Jenkins, Robert Brian.

In: Genes Chromosomes and Cancer, Vol. 48, No. 10, 10.2009, p. 854-864.

Research output: Contribution to journalArticle

Drucker, Kristen L. ; Kitange, Gaspar J. ; Kollmeyer, Thomas M. ; Law, Mark E. ; Passe, Sandra ; Rynearson, Amanda L. ; Blair, Hilary ; Soderberg, Cheryl L. ; Morlan, Bruce W. ; Ballman, Karla V. ; Giannini, Caterina ; Jenkins, Robert Brian. / Characterization and gene expression profiling in glioma cell lines with deletion of chromosome 19 before and after microcell-mediated restoration of normal human chromosome 19. In: Genes Chromosomes and Cancer. 2009 ; Vol. 48, No. 10. pp. 854-864.
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